山东大学耳鼻喉眼学报 ›› 2022, Vol. 36 ›› Issue (3): 165-170.doi: 10.6040/j.issn.1673-3770.0.2021.469

• 临床研究 • 上一篇    下一篇

慢性鼻窦炎伴双侧鼻息肉的发病与RANTES基因多态性的关系分析

刘厚军1,张倩2,程友1,薛飞1,许莉1,吴明海1   

  1. 1.东部战区总医院 耳鼻咽喉头颈外科, 江苏 南京 210000;
    2.河南大学第一附属医院 耳鼻咽喉头颈外科, 河南 开封 475000
  • 发布日期:2022-06-15
  • 通讯作者: 程友. E-mail:chengyou2002@126.com
  • 基金资助:
    2021年“科教强卫”项目-南京市医学重点专科(第十一周期)(SZDZK202002)

Relationships between RANTES gene polymorphisms and chronic sinusitis with bilateral nasal polyps

LIU Houjun1, ZHANG Qian2, CHENG You1, XUE Fei1, XU Li1, WU Minghai1   

  1. 1. Department of Otorhinolaryngology & Head and Neck Surgery, Eastern Theater Command General Hospital, Nanjing 210000, Jiangsu, China;
    2. Department of Otorhinolaryngology & Head and Neck Surgery, The First Affiliated Hospital of Henan, Kaifeng 250061, Henan, China
  • Published:2022-06-15

PDF (PC)

71

摘要： 目的 研究苏皖地区汉族人群中,RANTES基因(rs2107538和rs2280788)位点的单核苷酸多态性(SNP)与慢性鼻窦炎伴双侧鼻息肉(CRSwBNP)之间的相关性。 方法 分别收集CRSwBNP患者(病例组)、健康体检者(对照组)141例、108例,样本总量249例。收集所有样本的临床资料,并采集样本患者3 mL外周血,提取样本基因,采取病例-对照的实验方法,对RANTES基因位点(rs2107538和rs2280788)进行分型检测,最后使用Logistic回归分析法对分型结果进行统计分析。 结果 对病例组、对照组临床资料、实验数据进行统计学分析,发现性别与年龄分布无统计学差异(P>0.05)。研究发现rs2107538位点有纯合野生型CC、杂合型CT、纯合突变型TT三类基因型;而rs2280788位点有纯合突变型GG、纯合野生型CC、杂合型CG三类基因型。其中rs2107538位点等位基因T与野生型基因C比较(P=0.037),其增加了CRSwBNP患病风险。rs2280788位点的等位基因中未发现增加CRSwBNP的患病风险。对RANTES基因的两个位点进行连锁不平衡分析,结果发现,在单倍型RANTES rs2107538/rs2280788基因型中,基因型CG在CRSwBNP中起保护作用。 结论 RANTES基因的rs2280788位点多态性与CRSwBNP不存在相关性,而rs2107538位点多态性与CRSwBNP患者发病易感性相关。

关键词: RANTES基因, 单核苷酸多态性, 慢性鼻窦炎, 鼻息肉, 细胞因子

Abstract: Objective The aim of this study was to examine the relationship between single nucleotide polymorphisms of the RANTES gene(rs2107538 and rs2280788)and chronic sinusitis with bilateral nasal polyps(CRSwBNP)in the Han population of Jiangsu and Anhui regions. Methods One hundred and forty-one CRSwBNP patients(case group)and 108 healthy persons(control group)were enrolled, for a total of 249 participants. Clinical data were collected from all participants. Peripheral blood samples were collected and DNA was extracted. The RANTES gene locus(rs2107538 and rs2280788)was genotyped and the results were compared between case and control groups using logistic regression analysis. Results No significant differences in gender or age distribution were found between the case and control groups(P>0.05). CC, CT, and TT genotypes were detected at the rs2107538 locus and GG, CC, and CG genotypes were detected at the rs2280788 locus. The rs2107538 T allele was associated with a significantly increased risk of CRSwBNP(P=0.037, odds ratio［OR］=0.68, 95% confidence interval［CI］=0.47-0.98). The genotype at the rs2280788 locus was not associated with an increased risk of CRSwBNP. Linkage disequilibrium analysis showed that the RANTES rs2107538/rs2280788 C/G haplotype was protective against CRSwBNP(P=0.037, OR=0.68, 95%CI=0.47-0.98). Conclusion Thers2280788 polymorphism of the RANTES gene was not associated with CRSwBNP, but the rs2107538 polymorphism of the RANTES gene was associated with susceptibility to CRSwBNP.

Key words: RANTES gene, Single nucleotide polymorphism, Chronic sinusitis with bilateral nasal polyps, Cytokines

中图分类号: 

  • R765.25
[1] 杨一帆,程雷.儿童鼻窦炎与哮喘: 统一气道疾病[J].山东大学耳鼻喉眼学报, 2019, 33(6):10-15. doi: 10.6040 / j.issn.1673-3770.1.2019.061. YANG Yifan, CHENG Lei. Rhinosinusitis and asthma in children: united airw ay disease[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(6): 10-15. doi: 10.6040 / j.issn.1673-3770.1.2019.061.
[2] Maniu AA, Perde-Schrepler MI, Tatomir CB, et al. Latest advances in chronic rhinosinusitis with nasal polyps endotyping and biomarkers, and their significance for daily practice[J]. Rom J Morphol Embryol, 2020, 61(2):309-320. doi:10.47162/RJME.61.2.01.
[3] Lee TJ, Fu CH, Wang CH, et al. Impact of chronic rhinosinusitis on severe asthma patients[J]. PLoS One, 2017, 12(2): e0171047. doi: 10.1371/journal.pone.0171047.
[4] Bachert C, Gevaert P, Hellings P. Biotherapeutics in chronic rhinosinusitis with and without nasal polyps[J]. Allergy Clin Immunol Pract, 2017, 5(6): 1512-1516. doi: 10.1016/j.jaip.2017.04.024.
[5] De Greve G, Hellings PW, Fokkens WJ, et al. Endotype-driven treatment in chronic upperairw ay diseases[J]. ClinTransl Allergy, 2017, 7(1): 22. doi: 10.1186/s13601-017-0157-8.
[6] Shay AD, Taju deen BA. Histopathologic analysis in the diagnosis and management of chronic rhinosinusitis[J]. Curr Opin Otolaryngol Head Neck Surg, 2019, 27(1): 20-24. doi: 10.1097/MOO.0000000000000510.
[7] 陈鸣,俞雪飞.浅谈伴有变应性鼻炎的慢性鼻窦炎的治疗[J].山东大学耳鼻喉眼学报, 2018, 32(3):18-22. doi: 10.6040 / j.issn. 1673-3770.1.2018.007. CHEN Ming, YU Xuefei. Opinions on the treatment of chronic rhinosinusitis w ith allergic rhinitis[J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(3): 18-22. doi: 10.6040 / j.issn.1673-3770.1.2018.007.
[8] 安云崧,刘涛,周川,等. 单侧鼻息肉样病变的组织病理学及临床特征分析[J]. 中国耳鼻咽喉头颈外科,2020,27(10):586-588. doi:10.16066/j.1672-7002.2020.10.010.
[9] Eck hoff A, Cox D, Luk L, et al. Unilateral versus bilateral sinonasal disease: considerations in differential diagnosis and workup[J]. Laryngoscope, 2020, 130(4): 116-121. doi: 10.1002/lary.28108.
[10] Paz SM, Pinto JM, Corey JP, et al. Diagnostic algorithm for unilateral sinus disease: a 15-year retrospective review[J]. Int Forum Allergy Rhinol, 2015, 5(7): 590-596. doi: 10.1002/alr.21526.
[11] Drago L, Pignataro L, Torretta S. Microbiological aspects of acute and chronic pediatric rhinosinusitis[J]. J Clin Med, 2019, 8(2):149. doi:10.3390/jcm8020149.
[12] 王成硕,张罗.慢性鼻窦炎的治疗策略[J].临床耳鼻咽喉头颈外科杂志, 2016, 30(14): 1175-1178.doi:10.13201/j.issn.1001-1781.2016.14.024.
[13] 栾格,郝蕴,玄丽佳,等.嗜酸性粒细胞与慢性鼻-鼻窦炎伴鼻息肉发病机制相关性研究[J].国际耳鼻咽喉头颈外科杂志, 2018, 42(2): 98-101. doi:10.3760/cma.j.issn.1673-4106.2018.02.008.
[14] Mutuiri PM, Kutima HL, Mwapagha LM, et al. RANTES gene polymorphisms associated with HIV-1 infections in kenyan population[J]. Dis Markers, 2016: 4703854. doi: 10.1155/2016/4703854.
[15] Cerini F, Offord RE, Mcgowan I, et al. Stability of 5P12-RANTES, a candidate rectal microbicide, in human rectal lavage[J]. AIDS Res Hum Retroviruses, 2017, 33(8): 768-777. doi: 10.1089/AID.2016.0199. Epub 2017 Mar 6.
[16] 陈雪梅. 慢性鼻-鼻窦炎患者鼻息肉组织中CD69 RANTES的表达意义研究[J]. 河北医学, 2019, 25(10): 1674-1680. doi:10.3969/j.issn.1006-6233.2019.10.023.
[17] 徐亚男,陈冬,江祺川,等. RANTES、TSLP与EOS在鼻息肉组织中的表达及意义[J]. 锦州医科大学学报, 2017, 38(3):12-14. doi:10.13847/j.cnki.lnmu.2017.03.004.
[18] Rajarathnam K, Schnoor M, Richardson RM, et al. How do chemokines navigate neutrophils to the target site: Dissecting the structural mechanisms and signaling pathways[J]. Cell Signal, 2019,54:69-80. doi:10.1016/j.cellsig.2018.11.004.
[19] Zhang YQ, Gao XX. Association of RANTES gene polymorphisms with susceptibility to childhood asthma: a meta-analysis[J]. Medicine(Baltimore), 2020, 99(29):e20953. doi:10.1097/MD.0000000000020953.
[20] Wen D, Du X, Nie SP, et al. Association between RANTES gene polymorphisms and asthma: a meta-analysis[J]. PLoS One, 2014, 9(6):e90460. doi: 10.1371/journal.pone.0090460.
[21] Kaneko Y, Masuko H, Sakamoto T, et al. Asthma phenotypes in japanese adults-their associations with the CCL5 ADRB2 genotypes[J]. Allergology international: official journal of the Japanese Society of Allergology, 2013, 62(1):113-121. doi: 10.2332/allergolint.12-OA-0467.
[22] 刘全华,华丽,霍婧,等. 白介素13基因和RANTES基因多态性与汉族儿童哮喘发病关系研究[C] //.中华医学会2010年全国变态反应学术会议暨中欧变态反应高峰论坛参会指南/论文汇编,2010:94-95.
[23] 刘传合. 趋化因子单核苷酸多态性与中国北方汉族儿童哮喘易感性的相关性研究[D]. 北京:北京协和医学院(清华大学医学部)&中国医学科学院,2002.
[24] Szalai C, Kozma GT, Nagy A, et al. Polymorphism in the gene regulatory region of MCP-1 is associated with asthma susceptibility and severity[J]. J Allergy Clin Immunol, 2001, 108(3):375-381. doi: 10.1067/mai.2001.117930.
[25] Meyer-Hoffert U, Lezcano-Meza D, Bartels J, et al. Th2- and to a lesser extent Th1-type cytokines upregulate the production of both CXC(IL-8 and gro-alpha)and CC(RANTES, eotaxin, eotaxin-2, MCP-3 and MCP-4)chemokines in human airway epithelial cells[J]. Int Arch Allergy Immunol, 2003, 131(4):264-271. doi: 10.1159/000072138.
[26] Bachert C, Wagenmann M, Hauser U, et al. IL-5 synthesis is up-regulated in human nasal polyp tissue[J]. J Allergy Clin Immunol, 1997, 99:837-842. doi: 10.1016/s0091-6749(97)80019-x.
[27] Hamilos DL, Leung DY, Huston DP, et al. GM-CSF, IL-5 and RANTES immunoreactivity and mRNA expression in chronic hyperplastic sinusitis with nasal polyposis(NP)[J]. Clin Exp Allergy,1998, 28(9):1145-1152. doi: 10.1046/j.1365-2222.1998.00380.x.
[28] Choi YJ, Kang HN, Kim JH, et al.Differences in cytokine levels between the nasal polyps of adolescents with local allergic and adolescents with allergic or nonallergic rhinitis[J]. Allergy Asthma Proc, 2018, 39(4):e30-e37. doi:10.2500/aap.2018.39.4135.
[29] Xu M, Chen D, Zhou H, et al. The role of periostin in the occurrence and progression of eosinophilic chronic sinusitis with nasal polyps[J]. Sci Rep, 2017,7(1):9479. doi:10.1038/s41598-017-08375-2.
[1] 敖天, 程雷. 慢性鼻窦炎伴鼻息肉的内型研究及其指导下的精准控制与治疗[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 7-14.
[2] 熊攀辉,沈暘,杨玉成. 基于表型和内在型的慢性鼻窦炎诊治进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 15-19.
[3] 姚爽,娄鸿飞. 慢性鼻窦炎的内在型研究进展及精准医疗[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 20-29.
[4] 梁旭,史丽. 慢性鼻窦炎生物靶向药物治疗的研究进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 30-35.
[5] 石帅,郑泉,程雷. 度普利尤单抗在慢性鼻窦炎伴鼻息肉治疗中的研究进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 36-42.
[6] 王欢,胡俐,余洪猛. 慢性鼻窦炎相关嗅觉功能障碍研究进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 43-49.
[7] 宜若男,陈福权. 嗜酸性粒细胞与嗅觉功能障碍[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 50-55.
[8] 谷钰,万鑫,肖自安. 中性粒细胞和嗜酸性粒细胞在慢性鼻窦炎中的相互影响及临床治疗思考[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 56-63.
[9] 林海,朱莹,张维天. 慢性鼻窦炎发病中离子通道作用研究进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 64-70.
[10] 乔新杰,赵玉林. 慢性鼻窦炎中上皮间质转化信号转导通路及其他相关因子的研究进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 71-77.
[11] 黄丹怡,张婷,陈静,张薇. 上皮屏障在慢性鼻窦炎伴鼻息肉中的研究进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 78-83.
[12] 李佳倪,朱冬冬,孟粹达. 表观遗传学在慢性鼻窦炎伴鼻息肉发病机制中的作用[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 84-91.
[13] 于龙刚,姜彦. 鼻细菌微生物组与慢性鼻窦炎伴鼻息肉相关性的研究进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 92-97.
[14] 资昊坤,肖旭平,李云秋. 口服糖皮质激素在慢性鼻窦炎伴鼻息肉围手术期的应用现状[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 98-103.
[15] 曹轩,肖旭平,李云秋. 透明质酸在慢性鼻窦炎中的研究进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 104-109.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] 周斌,李滨 . 鼻内窥镜下鼻窦鼻息肉手术75例疗效观察[J]. 山东大学耳鼻喉眼学报, 2006, 20(1): 24 -26 .
[2] 张玉光,韩旭光,张华,王旭,徐湘辉 . 改良穿透性角膜移植术治疗真菌性角膜炎[J]. 山东大学耳鼻喉眼学报, 2006, 20(1): 94 -95 .
[3] 隆梅辉,何明强,牟艳云,田利健 . 上颌窦炎性肌纤维母细胞瘤1例并文献复习[J]. 山东大学耳鼻喉眼学报, 2008, 22(4): 329 -330 .
[4] 邹 俊,卢 奕,褚仁远 . 体外培养人胚晶状体上皮细胞生长特性的研究[J]. 山东大学耳鼻喉眼学报, 2008, 22(5): 453 -456 .
[5] 夏文清,郑 敏,满晓飞,李建平 . 手法劈核治疗老年性白内障[J]. 山东大学耳鼻喉眼学报, 2008, 22(5): 467 -469 .
[6] 李学昌,王金磊,张玉莉,董文汇,韩在文 . 中药冲洗对鼻黏膜纤毛超微结构的影响[J]. 山东大学耳鼻喉眼学报, 2006, 20(6): 522 -524 .
[7] 康宏建,李晓红,王保安,周 涛 . 重型颅脑损伤患者行气管切开术的意义[J]. 山东大学耳鼻喉眼学报, 2007, 21(3): 234 -236 .
[8] 闫 蕊,朱淋洁 . 翼状胬肉显微手术切除后角膜干细胞移植[J]. 山东大学耳鼻喉眼学报, 2007, 21(3): 243 -244 .
[9] 黄 方,朱从月 . p21、p73及PTEN在头颈部多原发癌中的表达及意义[J]. 山东大学耳鼻喉眼学报, 2007, 21(5): 388 -392 .
[10] 徐豪杰,李学忠,陈成芳,王学海 . 鼻内镜下鼻腔泪囊吻合术17例[J]. 山东大学耳鼻喉眼学报, 2008, 22(2): 132 -134 .
网站版权所有 © 2018 《山东大学耳鼻喉眼学报》编辑部　
地址：山东省济南市山大南路27号（250100）电话：+86-0531-88366912 E-mail: ebhxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发