Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2019, Vol. 33 ›› Issue (5): 96-100.doi: 10.6040/j.issn.1673-3770.0.2019.468

• Original Article • Previous Articles     Next Articles

Expression of the microRNA let-7a in the serum of 42 patients with laryngeal cancer and its significance

Lu LUO,En ZHOU,Si OUYANG,Yi CHEN,Xuping XIAO,Jihua WANG()   

  1. Department of Otolaryngology-Head and Neck Surgery, Hunan Provincial People′s Hospital, Changsha 41005, Hunan, China
  • Received:2018-10-22 Revised:2019-01-02 Online:2019-09-20 Published:2019-10-15
  • Contact: Jihua WANG E-mail:wangjihua29003@163.com

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Abstract: Objective

To investigate changes in the serum let-7a expression in laryngeal squamous cell carcinoma (LSCC) patients and the diagnostic value of let-7a for LSCC.

Methods

Forty-two LSCC patients and 42 healthy subjects who were hospitalized in our hospital were enrolled in this study. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to determine the expression level of let-7a in patient serum samples, the association between serum let-7a levels and clinical and pathological LSCC parameters, and the diagnostic efficacy of let-7a for LSCC.

 Results

Relative expression levels of let7a in the experimental (0.380±0.083) and normal control (0.933±0.0723) groups were significantly different . Relative let-7a expression levels in patients with laryngeal cancer before (0.380±0.0831) and after (0.488±0.1468) surgery were also significantly different. The let-7a level in tissues was associated with lymph node metastasis , but not with gender, age, smoking, alcohol consumption, tumor classification, infiltration depth, TNM stage, or degree of differentiation . ROC curve analysis showed that the best critical value for the relative expression of let-7a in 0.765 was laryngeal carcinoma with a sensitivity of 756.0%, a specificity of 76.1%, an AUC of 0.850, and a 95% confidence interval of 0.776-0.932.

 Conclusion

Overall, we confirmed that serum let-7a expression is down-regulated in LSCC patients and is a potential molecular marker for laryngeal cancer diagnosis. Our findings also showed that the serum let-7a level is associated with lymph node metastasis, which is helpful for tumor disease judgment and postoperative tumor monitoring, but further research is needed to validate these findings.

Key words: Laryngeal carcinoma, microRNA, Serum

CLC Number: 

  • R767.1

Fig.1

Serum miRNAlet-7a expression in laryngeal carcinoma patients and normal subjects"

Table 1

Relationship between the relative expression of serum miRNAlet-7a and clinicopathological parameters in patients with laryngeal cancer"

临床病理参数 总例数 let-7a表达量 t P
性别 男 38 0.381±0.087 0.158 0.876
女 4 0.374±0.042

年龄

（岁）

 ≤60 24 0.371±0.079 0.846 0.403
＞60 18 0.393±0.089
吸烟 吸烟 30 0.372±0.088 0.844 0.403
不吸烟 12 0.396±0.069
饮酒 饮酒 25 0.377±0.078 0.341 0.735
不饮酒 17 0.386±0.092
肿瘤分型 声门上型 9 0.371±0.052 0.413 0.682
声门型 33 0.384±0.090

淋巴结

转移

 无 35 0.393±0.081 2.250 0.03
有 7 0.319±0.070
浸润深度 T1~T2 38 0.377±0.084 0.747 0.459
T3~T4 4 0.41±0.084
TNM分期 I~II 33 0.388±0.080 1.118 0.27
III~IV 9 0.353±0.093
分化程度 中分化 6 0.380±0.088 0.220 0.827
高分化 36 0.384±0.029

Fig.2

ROC curve of serum miRNAlet-7a for laryngeal cancer diagnosis"

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