Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2024, Vol. 38 ›› Issue (4): 55-61.doi: 10.6040/j.issn.1673-3770.0.2023.146

• Original Article • Previous Articles    

Correlation analysis of gene expression profile of matrix metalloproteinases and their inhibitors in laryngeal squamous cell carcinoma and clinicopathological features

NI Rongsheng1, SHEN Xiaohui2, GAO Xia2   

  1. 1. Department of Otorhinolaryngology & Head and Neck Surgery, Nanjing Jiangbei Hospital Affiliated to Nantong University, Nanjing 210044, Jiangsu, China 2. Department of Otorhinolaryngology & Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu, China
  • Published:2024-07-09

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Abstract: Objective The study aimed to discover the gene expression profiles of the matrix metalloproteinases(MMPs)family and tissue inhibitors of matrix metalloproteinases(TIMPs)in laryngeal squamous cell carcinoma(LSCC), to analyze the genes most relevant to LSCC, and finally clarify their relationship with clinicopathological features. Methods Twenty-eight patients with LSCC were studied. In four patients, genes in the MMPs family and TIMPs that are up-regulated or down-regulated simultaneously were detected by a gene chip containing the entire human genome. The gene chip results were verified by reverse transcription-polymerase chain reaction(RT-PCR)and western blot in the other 24 patients. The correlation between the expression of the proteins encoded by the genes and the clinicopathological characteristics of the patients were investigated. Results The gene chip analysis revealed 14 differentially expressed genes of MMPs family between LSCC and adjacent normal cells. Of these, four genes were up-regulated, four were down-regulated, and the remaining six were up-regulated in some patients and down-regulated in others. No genes were consistently up- or down-regulated in the four patients. In contrast, all TIMPs were down-regulated between LSCC and adjacent normal cells. The TIMP4 gene was down-regulated in all four patients. RT-PCR and western blot results showed that the TIMP4 gene and protein was poorly expressed, respectively, in LSCC tissue. Western blot also showed that the expression of TIMP4 protein was correlated with lymph node metastasis. Conclusion To our knowledge, this is the first description of the expression profile of MMPs and TIMPs in LSCC. The expression of TIMP4 protein was associated with the clinicopathological features of LSCC. Future studies may further confirm TIMP4 protein as a predictor of the survival of patients with LSCC and/or a potential therapeutic target of LSCC.

Key words: Gene chip, Laryngeal squamous cell carcinoma, Matrix metalloproteinase, Tissue inhibitor of metalloproteinase

CLC Number: 

  • R739.65
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