JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (3): 76-80.doi: 10.6040/j.issn.1673-3770.0.2015.154

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In vitro study of the effect of Casein Kinase2 siRNA on anginogesis

DU Xiangge1, ZHANG Yingchun2, YAN Xin2, WANG Cui2, ZHAO Bojun2   

  1. 1. Second Clinical Medicine College, Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong, China;
    2. Department of Ophthalmology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2015-04-13 Revised:2015-05-13 Published:2015-06-16
  • Contact: 赵博军。E-mail:zhaobojun@hotmail.com E-mail:zhaobojun@hotmail.com

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Abstract: Objective To prove the effect of Casein Kinase2 siRNA on vascular endothelial growth factor(VEGF) expression and the angiogenic capacity in human umbilical vein endothelial cells (HUVECs). Methods HUVECs were transfected with either Casein Kinase2 siRNA or negative control siRNA. Cell proliferation was analyzed by MTT assay; the angiogenic capacity was accessed by tube formation; cell migration was assessed by the wound-healing model. Gene and protein expressions of VEGF were examined by Real-time PCR and Western blotting methods. Results Casein Kinase2 siRNA decreased the expressions of Casein Kinase2 and declined HUVEC proliferation, tube formation and migration. It was also reduced the expression of VEGF mRNA and VEGF protein. Conclusion Our results demonstrated that Casein Kinase2 siRNA reduced the expression of VEGF and declined the capacity of angiogenesis in HUVECs.

Key words: Angiogenesis, Vascular endothelial growth factor, Casein Kinase 2, siRNA, Vascular endothelial cells

CLC Number: 

  • R774.1
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