JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2016, Vol. 30 ›› Issue (4): 30-33.doi: 10.6040/j.issn.1673-3770.0.2016.162

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Effects of interferon gamma on Eosinophil apoptosis and Eotaxin levels in nasal lavage fluid of allergic rhinitis rats.

LI Qin1, CHEN Yanlin1, MA Yanyi1, ZHANG Yongdong   

  1. 1. Department of Otorhinolaryngology, Linyi Peoples Hospital, Linyi 276003, Shandong, China;2. Department of Phamacology, Shandong Medical College, Linyi 276002, Shandong, China
  • Received:2016-04-11 Online:2016-08-16 Published:2016-08-16

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Abstract: Objective To explore the effect of interferon gamma(IFN-γ)on the apoptosis of Eosinophils and Eotaxin levels from nasal lavage fluid in allergic rhinitis(AR)rats. Methods AR model was established by the sensitizer of ovalbumin and aluminum hydroxide. The 40 rats were randomly divided into the control group(group A, n=10), the allergic rhinitis group(group B, n=10), the budesonide group(group C, n=10)and the IFN-γ group(group D, n=10). In days 31 to 38 after the establishment of AR rats, each rat of the group D was administrated by instillation of 1 μg(1 μg/50 μL)IFN-γ into each of the nasal cavities one time a day, while each rat of the group B was administrated by instillation of 50 μL phosphate buffer in the same way, and each rat of the group C was administrated by instillation of 50 μL(1.28 μg/μL)budesonide in the same way. In days 39, the nasal lavage fluid was obtained to measure the apoptotic percentage of eosinophils and the apoptotic ratios were analyzed by flow cytometry. The levels of Eotaxin were detected by enzyme-linked immunosorbent assay(ELISA). Results The percentage of EOS in nasal lavage fluid of group C and group D was evidently lower than that of group B(all P<0.01). The eosinophil apoptotic ratio in group C and group D was evidently higher than that in group B(all P<0.01). The levels of Eotaxin in nasal lavage fluid of group C and group D were evidently lower than those in group B(all P<0.01);Furthermore significantly negative correlation was observed between the percentage of EOS and apoptotic ratio in the nasal lavage fluid of group D (r=-0.769,P<0.05). While significantly positive correlation was observed between the percentage of EOS and Eotaxin in the nasal lavage fluid of group D (r=0.750,P<0.05). Conclusion IFN-γcan significantly reduce the eosinophil infiltration and increase eosinophil apoptosis. It can reduce the levels of Eotaxin so as to achieve the purpose of treating allergic rhinitis.

Key words: Apoptosis, Nasal lavage fluid, Models, Allergic rhinitis, Eotaxin, Eosinophil, IFN-γ

CLC Number: 

  • R765.21
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