JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2017, Vol. 31 ›› Issue (5): 72-78.doi: 10.6040/j.issn.1673-3770.0.2017.297

Previous Articles     Next Articles

Investigation of chemosensitization induced by nitric oxide on nasopharyngeal carcinoma CNE-2 cells.

ZHANG Caixia1,2, LIU Yangyun1, JIANG Wen1, LIU Gengxun1, CAO Hang1, CHEN Qiong1, ZHANG Jishuai1   

  1. Department of Otolaryngology, 1. No.163 Hospital of PLA /The Second Affiliated Hospital of Hunan Normal University Medical College, Changsha 410003, Hunan, China;2. Liuzhou Workers Hospital / Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou 545000, Guangxi, China
  • Received:2017-07-07 Online:2017-10-16 Published:2017-10-16

PDF (PC)

394

Abstract: Objective To investigate whether exogenous nitric oxide(NO)could increase chemosensitization to cisplatin(DDP)in CNE2 cells, and to provide an experimental and theoretical basis for improving the effectiveness of chemotherapy for nasopharyngeal carcinoma. Methods CNE-2 cells were treated with various concentrations of sodium nitroprusside(SNP), DDP, and both chemicals. The nitrate reductase method was used to detect the concentration of NO. Morphological changes of cells were observed using an inverted phase contrast microscope and CCK8 assays were used to examine the viability of cells. Flow cytometry was applied to detect apoptosis in NPC cells. Results (1)The concentration of NO was positively correlated with the concentration of SNP, and this correlation was statistically significant(r=0.968, P<0.05).(2)Compared with the CNE-2 cells in the SNP group, DDP group, and control group, the CNE-2 cells in the DDP+SNP group showed more extensive morphological changes.(3)The inhibitory effects of SNP+DDP were significantly greater than those of SNP or DDP alone(P<0.05).(4)Compared with the groups treated with SNP or DDP individually, flow cytometry showed that the apoptosis rate of CNE-2 cells was significantly higher in the group treated with both SNP and DDP(P<0.05). Conclusion Exogenous NO can inhibit CNE-2 proliferation, and the inhibitory effect was positively correlated with the concentration of NO. The proper concentration of exogenous NO can significantly enhance the chemosensitivity of CNE-2 cells to DDP, without substantial toxicity.

Key words: Nasopharyngeal carcinoma, Sodium nitroprusside, Cis-platinum, Apoptosis, Chemosensitization, Nitric oxide

CLC Number: 

  • R739.62
[1] Tan G, Tang X, Tang F. The role of microRNAs in nasopharyngeal carcinoma[J]. Tumour Biol, 2015, 36(1):69-79.
[2] Razak AR, Siu LL, Liu FF, et al. Nasopharyngeal carcinoma: the next challenges[J]. Eur J Cancer, 2010, 46(11):1967-1978.
[3] Lee AW, Lin JC, Ng WT. Current management of nasopharyngeal cancer[J]. Semin Radiat Oncol, 2012, 22(3):233-244.
[4] Perri F, Bosso D, Buonerba C, et al. Locally advanced nasopharyngeal carcinoma: current and emerging treatment strategies[J]. World J Clin Oncol, 2011, 2(12):377-383.
[5] Zhang L, Chen QY, Liu H, et al. Emerging treatment options for nasopharyngeal carcinoma[J]. Drug Des Devel Ther, 2013, 7(2):37-52.
[6] 张彩霞, 刘阳云. 一氧化氮对肿瘤细胞化疗增敏机制研究进展[J]. 临床与病理杂志,2016,36(8):1224-1229. ZHANG Caixia, LIU Yangyun. Research progress of nitric oxide chemosensitization mechanisms on tumor cells[J]. Inter J Pathol Clin Med, 2013, 7(2):37-52.
[7] Yang L, Lan C, Fang Y, et al. Sodium nitroprusside(SNP)sensitizes human gastric cancer cells to TRAIL-induced apoptosis[J]. Int Immunopharmacol, 2013, 17(2):383-389.
[8] Seabra AB, de Lima R, Calderón M. Nitric oxide releasing nanomaterials for cancer treatment: current status and perspectives[J]. Curr Top Med Chem, 2015, 15(4):298-308.
[9] Zhang HX, Deng C, Liu OS, et al. Inducible nitric oxide inhibitor enhances the anti-tumor effect of cisplatin on CNE-2 cells by inducing cell apoptosis[J]. Eur Rev Med Pharmacol Sci, 2014, 18(19):2789-2797.
[10] 刘阳云, 赵素萍, 刘庚勋, 等. 诱导型一氧化氮合酶在指导晚期鼻咽癌治疗方案选择中的价值[J]. 中国癌症杂志,2007,17(9):684-688. LIU Yangyun, ZHAO Suping, LIU Gengxun, et al. The value of inducible nitric oxide synthase to select the project of treatment in nasopharyngeal carcinoma[J]. Chin Oncol, 2007, 17(9):684-688.
[11] 刘阳云, 赵素萍, 刘庚勋, 等. 诱导型一氧化氮合酶与环氧合酶-2在鼻咽癌中表达的初步研究[J]. 中华放射肿瘤学杂志,2007,16(2):155-156.
[12] Mocellin S. Nitric oxide: cancer target or anticancer agent?[J]. Curr Cancer Drug Targets, 2009, 9(2):214-236.
[13] 张彩霞,刘阳云. 一氧化氮供体型化合物化疗增敏效应[J]. 国际肿瘤学杂志,2016,43(9):53-56. ZHANG Caixia, LIU Yangyun. Neoplasms chemosensitization enhancing effects of nitric oxide donor compounds[J]. J Int Oncol, 2016, 43(9):53-56.
[14] Hirst D, Robson T. Nitric oxide in cancer therapeutics: interaction with cytotoxic chemotherapy[J]. Curr Pharm Des, 2010, 16(4):411-420.
[15] Carradori S, Mollica A, Monte CD, et al. Nitric oxide donors and selective carbonic anhydrase inhibitors: a dual pharmacological approach for the treatment of glaucoma, cancer and osteoporosis[J]. Molecules, 2015, 20(4):5667-5679.
[16] Reynolds MM, Witzeling SD, Damodaran VB, et al. Applications for nitric oxide in halting proliferation of tumor cells[J]. Biochem Biophys Res Commun, 2013, 4(31):647-651.
[17] Persson PB, Bondke Persson A. Nitric oxide: a classic revisited[J]. Acta Physiol, 2013, 207(3):427-429.
[18] Aranda E, López-Pedrera C, De La Haba-Rodriguez JR, et al. Nitric oxide and cancer: the emerging role of S-nitrosylation[J]. Curr Mol Med, 2012, 12(1):50-67.
[19] Singh S, Gupta AK. Nitric oxide: role in tumour biology and iNOS/NO-based anticancer therapies[J]. Cancer Chemother Pharmacol, 2011, 67(6):1211-1224.
[20] González R1, Ferrín G, Aguilar-Melero P, et al. Targeting hepatoma using nitric oxide donor strategies[J]. Antioxid Redox Signal, 2013, 18(5):491-506.
[21] Huerta-Yepez S, Baritaki S, Baay-Guzman G, et al. Contribution of either YY1 or Bcl XL-induced inhibition by the NO-donor DETANONOate in the reversal of drug resistance, both in vitro and in vivo. YY1 and BclXL are overexpressed in prostate cancer[J]. Nitric Oxide Soc, 2013, 29(1):17-24.
[22] Bian K, Murad F. sGC-cGMP signaling: target for anticancer therapy[J]. Adv Exp Med Biol, 2014, 14(8):5-13.
[23] Bian K, Murad F. What is next in nitric oxide research? From cardiovascularsystem to cancer biology[J]. Nitric Oxide, 2014, 4(3):3-7.
[24] Bonavida B, Garban H. Nitric oxide-mediated sensitization of resistant tumor cells to apoptosis by chemo-immunotherapeutics[J]. Redox Biol, 2015, 10(6):486-494.
[25] Sarti P, Forte E, Mastronicola D, et al. Cytochrome c oxidaseand nitric oxide in action: molecular mechanisms and pathophysiological implications[J]. Biochim Biophys Acta, 2012, 18(7):610-619.
[26] Bonavida B, Baritaki S. The novel role of yin yang 1 in the regulation of epithelial to mesenchymal transition in cancer via the dysregulated NF-κB/Snail/YY1/RKIP/PTEN circuitry[J]. Crit Rev Oncog, 2011, 16(4):211-226.
[27] Baritaki S, Huerta-Yepez S, Sahakyan A, et al. Mechanisms of nitric oxide-mediated inhibition of EMT in cancer: inhibition of the metastasis-inducer snail and induction of the metastasis-suppressor RKIP[J]. Cell Cycle, 2010, 12(9):4931-4940.
[28] Tang H, Park S, Sun SC, et al. Kolcgnaling components of the IκB kinase complex[J]. FEBS Lett, 2010, 5(8):662-668.
[29] Domingo E, Church DN, Sieber O, et al. Evaluation of PIK3 CA mutation as a predictor of benefit from nonsteroidal anti-inflammatory drug therapy in colorectal cancer[J]. J Clin Oncol, 2013, 31(4):4297-4234.
[1] SU Jie, AI Xin, MA Chunmei, YANG Fuyu, HUANG Shuai. Relationship between acute intraocular hypertension and lateral geniculate neuron autophagy and apoptosis in rats [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 68-71.
[2] ZHAO Xiaoyan, WU Caiqin, REN Xiaoyong, WANG Zhenghui. A polysaccharide from hedyotis diffusa induces apoptosis and inhibits migration in Hep-2 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(2): 84-87.
[3] ZHOU Yibo, GONG Xiaorong, YU Feng. Effects of miR-150 targeted to Nanog on proliferation and invasion of nasopharyngeal carcinoma side population cells. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(5): 79-84.
[4] DU Zhihong. Application of oro-nasal combination approach under electronic nasopharyngoscope in the nasopharyngeal biopsy. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(4): 117-118.
[5] . Effects of concurrent chemotherapy and radiotherapy on the quality of life in children and adolescents with nasopharyngeal carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(2): 55-58.
[6] . Construction of c-Met target chimeric antigen receptor T cells and evaluation the role on nasopharyngeal carcinoma cells. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(2): 49-54.
[7] . Curcumin combined with resveratrol enhances their anti-tumoral effects in the head and neck carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(2): 67-72.
[8] . Efficacy of recombinant human epidermal growth factor in prevention of radioactive oral mucosa inflammation and acute radiation dermatitis for nasopharyngeal carcinoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(1): 95-99.
[9] WANG Yaning, GENG Bo, LI Baiyan, CUI Chaoyang, WANG Qirong. The treatment experience on osteoradionecrosis of skull base with nasopharyngeal carcinoma after radiotherapy. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(6): 42-45.
[10] LIU Ye, SUN Dianshui, YIN Jinjun, XIA Ming, XU Ying, SUN Jianhua. Vestibular function damage mechanisms in nasopharyngeal carcinoma patients after intensity-modulated radiotherapy. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(6): 14-17.
[11] LI Qin, CHEN Yanlin, MA Yanyi, ZHANG Yongdong. Effects of interferon gamma on Eosinophil apoptosis and Eotaxin levels in nasal lavage fluid of allergic rhinitis rats. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(4): 30-33.
[12] WANG Guixiang, WANG Pengpeng, TANG Lixing, ZHANG Yamei, ZHANG Jie, GE Wentong.. Study on measurement of nasal nitric oxide in children with allergic rhinitis. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(1): 14-16.
[13] WANG Junying, LI Xiaofeng. Effect of deproteinized extract of calf blood gel eye on the injury of corneal endothelial cells after nasopharyngeal carcinoma radiotherapy. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(1): 76-79.
[14] ZHAO Yanlian, LU Yongtian, YANG Jihong, ZHANG Juan, MIAO Fangfang, LI Jieping. Relationship between the expression of EGFL7 in nasopharyngeal carcinoma and the invasion and metastasis of tumor [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(6): 31-35.
[15] CHE Juan, ZHAO Hongchun, LI Jinling, WANG Yanfei. The relationship between D-Dimer and nasopharyngeal carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(5): 47-49.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd