Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2025, Vol. 39 ›› Issue (1): 61-67.doi: 10.6040/j.issn.1673-3770.0.2024.027

• Original Article • Previous Articles    

Effect of Crocin on Type 2 inflammation in eosinophilic chronic rhinosinusitis with nasal polyps

ZHANG Jie1,2, NIMA Jizong2, XU Xiaodong3, ZHOU Jing4, LIU Jianmin4, LUO Yirui1,2, DU Jintao4, BA Luo1,2   

  1. 1. Medical College of Tibet University, Lasa 850000, Xizang, China 2. Department of Otorhinolaryngology, People's Hospital of Tibet Autonomous Region, Lasa 850000, Xizang, China3. Department of Otolaryngology, Shannan City People's Hospital, Shannan 856000, Xizang, China4. Department of Otorhinolaryngology & Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan,  China
  • Published:2025-01-17

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Abstract: Objective To study the effect of crocin on Eosinophilic Chronic Rhinosinusitis with Nasal Polyps, the regulatory role and mechanism of Peripheral blood mononuclear cell(PBMC)type 2 inflammation in patients with Eos CRSwNP, providing a new target or pathway for precise treatment of nasal polyps. Methods Chronic rhinosinusitis with nasal polyps(CRSwNP)patients were collected for pathological diagnosis. Eosinophilic Chronic Rhinosinusitis with Nasal Polyps(Eos CRSwNP)and Non-eosinophilic Chronic Rhinosinusitis with Nasal Polyps(Non-Eos CRSwNP)were classified according to the inflammatory cell types. PBMC in peripheral blood of Eos CRSwNP patients was extracted by density gradient centrifugation. PBMC was stimulated with different concentrations of IL-33, and transcription factors GATA3, T-bet, and cell receptor CRTH2 were detected by qPCR. The expression of inflammatory cytokines INF-γ, IL-5, and IL-13 was analyzed by qPCR and ELISA. Then different concentrations of crocin were added for intervention, and GATA3, T-bet, and CRTH2 were detected by Western blotting and qPCR. GraphPad Prism software was used for statistical analysis to compare whether there were significant differences in index changes before and after IL-33 stimulation and crocin intervention. Results A total of 16 cases were pathologically confirmed as Eos CRSwNP. After stimulating PBMC with different concentrations of IL-33, qPCR confirmed that the expression of T-bet, GATA3, and CRTH2 was significantly increased(P values were 0.004, 0.001, and 0.026, respectively). qPCR and ELISA further revealed upregulation of cytokines INF-γ, IL-5, and IL-13(P values of qPCR were 0.001, 0.001, and 0.003, respectively; P values of ELISA were 0.035, 0.006, and 0.001, respectively), with the most significant activation of PBMC inflammatory cells observed at an IL-33 concentration of 20 ng/mL. After intervention with crocin, Western blotting and qPCR showed a downward trend in the expression of T-bet, GATA3, and CRTH2, with the most significant effect observed at a concentration of 10 μg/mL(P values of WB were 0.027, 0.001, and 0.002, respectively; P values of qPCR were 0.019, 0.001, and 0.001, respectively). Conclusion Crocin can significantly inhibit type 2 inflammation in IL-33-activated CRSwNP patients and has potential value in the development of new therapeutic drugs for CRSwNP.

Key words: Chronic rhinosinusitis, Nasal polyps, Eosinophils, Peripheral blood mononuclear cells, Crocin, Type 2 inflammatory effect

CLC Number: 

  • R765.41
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