关键词: 喉, 器官移植, 动物模型, 分期, 再植

Abstract: Objective To establish a model of cyno-laryngeal transplantation. Methods 10 beagles, matched-pairs in homogeneity and weight (8-10kg), were divided into 5 groups with allotransplantation. In stage Ⅰ, vascular circulation was managed with an end-to-end or end-to-lateral anastomosis of the allograft bilateral common carotid arteries with the recipient bilateral common carotid arteries, and bilateral external jugular veins. The recurrent laryngeal nerve was anastomosed end-to-end. Then, a vasculared allograft was embeded in the cervical subcutaneous of the receptor, and an immuno-depressant (cosA) was administered to the receptor based on the weight every day. In stage Ⅱ, the allograft was reimplanted with the pharyngeal and the trachea portion anastomosed when the allograft survived. Results In stage Ⅰ, the allograft survived in groups 3, 4 and 5 for up to one month and it had necrosis in groups 1 and 2 for receptor cervical infection. Conclusions The receptor beagles can tolerance the laryngeal allotransplantation by stages (laryngeal allograft fore-embed model) and the allograft can survive, however, glandular organ secretion is a troublesome problem and an immuno- long time.

Key words: Laryngeal, Allotransplantation, Model, animal, Stages, Reimplantation