JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (2): 48-51.doi: 10.6040/j.issn.1673-3770.0.2014.369

Previous Articles     Next Articles

Expression of TSLP and its relationship with the infiltrsting regulatory T cells in chronic rhinosinusitis

ZHU Hui-tao, CAI Xiao-lan, FENG Xin, QI Jun-jun, ZHANG Jin-ling, MA Yue, LI Xue-zhong   

  1. Department of Otolaryng, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-11-19 Online:2015-04-16 Published:2015-04-16
  • Contact: 李学忠。E-mail:lxzebyh@163.com E-mail:lxzebyh@163.com

PDF (PC)

1884

Abstract: Objective To determine the expression of thymic stromal lymphopoietin(TSLP)and regulatory T cells(CD4+CD25+Foxp3+Treg) in human chronic rhinosinusitis(CRS) and to analyze their pathogenesis in CRS. Methods The sinus mucosa from 25 patients with CRS was studied. Inferior nasal turbinate from 28 patients with nasal septum deviation served as the control. Their histological characteristics were analyzed by HE stain. The expressions of TSLP and Foxp3 mRNA were detected by Q-RT-PCR. Immunohistochemistry was used to detect CD4+CD25+Foxp3+Treg cells. The correlation of TSLP with the number of Tregs and the correlation of TSLP with Foxp3 mRNA were evaluated. Results The expressions of TSLP mRNA, Foxp3 mRNA and Treg cells were significant higher in CRS compared with the control group(P<0.05). There was positive correlation between TSLP and Foxp3 mRNA(r=0.977 3, P<0.000 1). At the same time, there was positive correlation between TSLP and the number of Tregs(r=0.864 6, P<0.0001). Conclusion TSLP could play an important role in CRS by inducing Treg cells.

Key words: Thymic stromal lymphopoietin, Infiltrasting regulatory T cells, Foxp3 gene, Chronic rhinosinusitis

CLC Number: 

  • R765.4
[1] Watanabe N, Wang Y H, Lee H K, et al. Hassall's corpuscles instruct dendritic cells to induce CD4+ CD25+ regulatory T cells in human thymus[J]. Nature, 2005, 436(7054):1181-1185.
[2] Fontenot J D, Gavin M A, Rudensky A Y. Foxp3 programs the development and function of CD4+ CD25+ regulatory T cells[J]. Nature Immunol, 2003, 4(4):330-336.
[3] Nagarkar D R, Poposki J A, Tan B K, et al. Thymic stromal lymphopoietin activity is increased in nasal polyps of patients with chronic rhinosinusitis[J]. J Allergy Clin Immunol, 2013, 132(3):593-600. e12.
[4] Umetsu D T, DeKruyff R H. The regulation of allergy and asthma[J]. Immunological Review, 2006, 212(1):238-255.
[5] Mills K H G, McGuirk P. Antigen-specific regulatory T cells-their induction and role in infection[C]//Seminars in immunology. Academic Press, 2004, 16(2):107-117.
[6] Walker M R, Carson B D, Nepom G T, et al. De novo generation of antigen-specific CD4+ CD25+ regulatory T cells from human CD4+ CD25-cells[J]. Proc Natl Acad Sci U S A, 2005, 102(11):4103-4108.
[7] von Boehmer H. Selection of the T-cell repertoire: receptor-controlled checkpoints in T-cell development[J]. Adv Immunol, 2004, 84(2):201-238.
[8] Josefowicz S Z, Rudensky A. Control of regulatory T cell lineage commitment and maintenance[J]. Immunity, 2009, 30(5):616-625.
[9] Kim Y M, Munoz A, Hwang P H, et al. Migration of regulatory T cells toward airway epithelial cells is impaired in chronic rhinosinusitis with nasal polyposis[J]. Clin Immunol, 2010, 137(1):111-121.
[10] Reche P A, Soumelis V, Gorman D M, et al. Human thymic stromal lymphopoietin preferentially stimulates myeloid cells[J]. J Immunol, 2001, 167(1):336-343.
[11] Caretto D, Katzman S D, Villarino A V, et al. Cutting edge: the Th1 response inhibits the generation of peripheral regulatory T cells[J]. J Immunol, 2010, 184(1):30-34.
[12] Zhou L, Chong M M W, Littman D R. Plasticity of CD4+ T cell lineage differentiation[J]. Immunity, 2009, 30(5):646-655.
[1] FENG Sicong, YU Xiaolan, LOU Dan. Evaluation of the effect of Messerklinger middle turbinate plasty on chronic rhinosinusitis with nasal polyps based on the expression of Ki67 and GM-CSF in vesicular tissue [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(3): 31-39.
[2] ZHAI Xuechun, BIAN Xin, CHEN Jingcai, DENG Jiayu, YE Zi, YANG Pingli. Characteristics and influencing factors of anxiety and depression in patients with chronic frontal sinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(2): 35-43.
[3] LI Yangsong, YUAN Fayang, YANG Yan, ZHANG Tian, YU Guodong. Bibliometric analysis of biologic agents for the treatment of chronic rhinosinusitis based on the Web of Science database [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 54-64.
[4] ZHANG Jiaqi, YUAN Ye, HONG Chen, GU Min, CHENG Lei, LU Meiping. Mendelian randomization study of gut microbiota, chronic sinusitis, and nasal polyps: Causal relationships and metabolite-mediated effects [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 49-60.
[5] ZHANG Guangling, CHEN Xingxue, WU Tianyi, SUN Zhanwei, WANG Weiwei, LI Shichao,WANG Guangke. The expression and function of Tespa1 in chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 35-42.
[6] CAO Zhengyong, LI Xiaobo. Comparison of the safety and efficacy of short-course postoperative topical glucocorticoid adjuvant therapy for eCRSwNP combined with asthma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 43-50.
[7] WANG Aowei, SHI Wenjie. Study on correlation between traditional Chinese medicine syndrome types and TFH cell related factors in CRSwNP [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 54-60.
[8] ZHANG Jie, NIMA Jizong, XU Xiaodong, ZHOU Jing, LIU Jianmin, LUO Yirui, DU Jintao, BA Luo. Effect of Crocin on Type 2 inflammation in eosinophilic chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 61-67.
[9] LI Ting, ZHAO Chong, WU Xian. The relationship between miR-34a expression in nasal mucosa tissue and postoperative recurrence in patients with chronic sinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(5): 20-25.
[10] CHEN Xingxue, ZHANG Guangling, WU Tianyi, WANG Weiwei, SUN Zhanwei, LI Shichao, WANG Guangke. Analysis of anti-IL-4Rα monoclonal antibody and endoscopic sinus surgery in the treatment of eosinophilic chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(4): 43-54.
[11] ZHANG Shihan, LIU Hongbing. Effect of matrix metalloproteinase on tissue remodeling in chronic rhinosinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(3): 116-123.
[12] WANG Xiaoai, ZHANG Qianqian, CHENG Xiangyu, LI Zhipeng, ZHANG Weitian, YE Haibo. A clinical efficacy analysis of vidian neurectomy in the treatment of type 2 chronic rhinosinusitis with allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(5): 42-49.
[13] ZHU Yu, ZHU Xinhua. Research progress on the role of TH2 cytokines in Type2 chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(5): 156-161.
[14] AO Tian,CHENG Lei. An endotype study of chronic rhinosinusitis with nasal polyps and precise control and treatment under the guidance [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 7-14.
[15] YAO Shuang,LOU Hongfei. Advances in endotypes and precision medicine in chronic rhinosinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 20-29.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd