JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2015, Vol. 29 ›› Issue (2): 74-80.doi: 10.6040/j.issn.1673-3770.0.2014.351

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Effect of nicotine on human RPE cells and HUVECs

ZHANG Ying-chun, DU Xiang-ge, YAN Xin, WANG Cui, ZHAO Bo-jun   

  1. Department of Ophthalmology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2014-11-05 Online:2015-04-16 Published:2015-04-16
  • Contact: 赵博军。E-mail:zhaobojun@hotmail.com E-mail:zhaobojun@hotmail.com

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Abstract: Objective To evaluate the influences of Nicotine (NT) from cigarette smoke on VEGF and PEDF expressions in retinal pigment epithelium (RPE) cells and human umbilical vein endothelial cells (HUVECs). In addition, the angiogenic behaviors of endothelial cells with NT treatment were assessed by using in vitro methods. Methods ARPE-19 cells and HUVECs were treated with different concentrations of NT for a period of times. Proliferative effect was investigated by using the method of MTT analysis. HUVECs migration and tube formation were assessed by using the wound-healing and Matrigel models. The expressions of VEGF and PEDF in both types of cells were examined by real-time PCR and Western blotting. Results There was no proliferation of ARPE-19 cells following treatment with various concentrations of NT. However, NT significantly stimulated HUVECs proliferation, migration, and tube formation. NT up-regulated the expression of VEGF but suppressed the expression of PEDF at both mRNA and protein levels in a dose- and time-dependent manner in ARPE-19 cells and HUVECs. Conclusion Our results demonstrate that NT promoted endothelial cellular proliferation, migration and angiogenesis of HUVECs in vitro. These effects might partly play through simultaneous modulation of VEGF/PEDF signaling in ARPE-19 cells and HUVECs.

Key words: Nicotine, Vascular endothelial growth factor, Pigment epithelium-derived factor, Vascular endothelial cells, Choroidal neovascularization, Human retinal pigment epithelium cells

CLC Number: 

  • R774.1
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