梅尼埃病与前庭性偏头痛共表达基因特征及天麻素的分子对接预测

doi:10.6040/j.issn.1673-3770.0.2024.315

摘要/Abstract

摘要： 目的探讨运用生物信息学分析方法对梅尼埃病(meniere's disease, MD)和前庭性偏头痛(vestibular migraine, VM)的共表达基因特征进行初步探索,筛选核心共表达基因。利用分子对接技术预测天麻素对核心共表达基因的治疗潜力。方法从GEO数据库得到MD和VM的微阵列数据并进行加权基因共表达网络分析(weighted gene co-expression network analysis, WGCNA)和差异表达基因(differentially expressed genes, DEGs)分析。通过CTD和GeneCards公共数据库筛选MD和VM共表达基因进行补充。运用DAVID数据库进行基因本体(gene ontology, GO)和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes, KEGG)富集分析。通过STRING在线数据库构建的蛋白质互作网络(protein-protein interaction networks, PPI)筛选核心共表达基因。将天麻素与核心共表达基因进行分子对接预测。结果共获得92个MD和VM共表达基因。富集分析显示两种疾病都表现出显著的免疫和炎症反应病理特征。MD和VM共同病理机制可能与TNF、IL-17和NF-kB信号通路有关。6个核心共表达基因有TNF、IL-1Β、IL6、ITGAM、CXCL10和MMP9,它们同样具有免疫炎症特征。天麻素与它们的结合亲和力分别为-6.59、-6.50、-4.80、-6.00、-5.59和-8.47 kcal/moL。结论免疫和炎症反应是MD和VM的共同病理基础,支持这两个疾病之间的相关性。TNF和NF-kB信号通路可能在共同病理机制中发挥重要作用。TNF、IL-1Β、IL6、CXCL10和MMP9可能是MD和VM潜在的共同诊断和治疗靶点。天麻素与它们结合力较强,具有一定治疗潜力。

关键词: 梅尼埃病, 前庭偏头痛, 共表达基因特征, 天麻素, 分子对接

Abstract: Objective To explore the co-expression gene characteristics of meniere's disease(MD)and vestibular migraine(VM)by bioinformatics analysis, and screen the core co-expression genes. Molecular docking technique was used to predict the therapeutic potential of gastrodin on core coexpressed genes. Methods The microarray data of MD and VM were obtained from the GEO database for the weighted gene co-expression network analysis(WGCNA)and differentially expressed genes(DEGs)analysis. The co-expressed genes of MD and VM were supplemented by screening the CTD and GeneCards public databases. The DAVID database was used for Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis. The core co-expressed genes were screened through the protein-protein interaction networks(PPIs)constructed from the STRING online database. moLecular docking prediction was performed between gastrodin and the core co-expressed genes. Results A total of 92 MD and VM co-expressing genes were obtained. Enrichment analysis showed that both diseases share significant pathological features of immune and inflammatory responses. The common pathological mechanism of MD and VM may be related to TNF, IL-17 and NF-kB signaling pathways. The six core co-expressed genes are TNF, IL-1Β, IL6, ITGAM, CXCL10 and MMP9, which also have immuno-inflammatory properties. The binding affinities of gastrodin to these genes were -6.59, -6.50, -4.80, -6.00, -5.59 and -8.47 kcal/moL respectively. Conclusion Immune and inflammatory responses are the common pathological basis of MD and VM, supporting the association between the two diseases.TNF and NF-kB signalling pathways may play important roles in common pathological mechanisms. TNF, IL-1Β, IL6, CXCL10, and MMP9 may be potential common diagnostic and therapeutic targets for MD and VM. Gastrodin binds strongly to these and has some therapeutic potential.

Key words: Meniere's disease, Vestibular migraine, Shared gene signatures, Molecular docking, Gastrodin

中图分类号:

R764.4

王博深,冷辉,孙海波. 梅尼埃病与前庭性偏头痛共表达基因特征及天麻素的分子对接预测[J]. 山东大学耳鼻喉眼学报, 2025, 39(1): 29-40.

WANG Boshen, LENG Hui, SUN Haibo. Co-expressing genes in Meniere's disease and vestibular migraine and molecular docking prediction of gastrodin[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 29-40.

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