Foxp3基因转染的Teff细胞抗高危角膜移植排斥的作用

doi:10.6040/j.issn.1673-3770.0.2014.272

山东大学耳鼻喉眼学报 ›› 2015, Vol. 29 ›› Issue (1): 44-48.doi: 10.6040/j.issn.1673-3770.0.2014.272

Foxp3基因转染的Teff细胞抗高危角膜移植排斥的作用

赵军^1,2, 刘景¹, 王大江³, 陈国玲¹, 王琪², 张晗¹

1. 山东大学第二医院眼科, 山东济南 250033;
2. 辽河油田总医院眼科, 辽宁盘锦 124010;
3. 解放军总医院眼科, 北京 100853

收稿日期:2014-08-17 出版日期:2015-02-16 发布日期:2015-02-16
通讯作者: 张晗,教授。E-mail:zhanghan9157@gmail.com E-mail:zhanghan9157@gmail.com
作者简介:赵军。E-mail:zhaojun7837985@sina.com
基金资助:
山东科技厅博士基金(2008BS03049)

Allograft rejection after high risk corneal transplantation with Foxp3 Gene-transferred effective T cells

ZHAO Jun^{1 2}, LIU Jing¹, WANG Da-jiang³, CHEN Guo-ling¹, WANG Qi², ZHANG Han¹

1. Liaohe Oilfield General Hospital, Panjin 124010, Liaoning, China;
2. The Second Hospital of Shandong University, Jinan 250033, Shandong, China;
3. Chinese PLA General Hospital, Beijing 100853, China

Received:2014-08-17 Online:2015-02-16 Published:2015-02-16

摘要/Abstract

摘要： 目的探讨Foxp3基因转染的Teff淋巴细胞在抗高危角膜移植排斥中的作用。方法将Foxp3基因转染Teff淋巴细胞与Teff淋巴细胞进行混合培养,观察其对Teff淋巴细胞的抑制作用;角膜缝线法诱导Balb/c小鼠产生角膜新生血管,角膜缝线1周后以Balb/c小鼠为受体,以C57/BL6小鼠为供体行穿透性角膜移植,术前1 d尾静脉输入Foxp3基因转染的Teff淋巴细胞。术后,每天观察角膜植片排斥情况;每两周尾静脉取血观察淋巴细胞分型。结果体外实验显示Foxp3基因转染的Teff淋巴细胞对Teff淋巴细胞具有明显抑制作用;在高危角膜移植小鼠,Foxp3基因转染的Teff淋巴细胞可适当延长植片存活时间(19.71±4.19)d, Treg组角膜植片存活时间(19.28±4.46)d,两组对比差异无统计学意义(P>0.05);空白组角膜存活时间(15.57±2.22),与Foxp3基因转染的Teff细胞组对比,差异有统计学意义(P<0.05);流式细胞学检查显示:FoxP3转染的淋巴细胞可延缓CD4+T淋巴细胞与CD8+T淋巴细胞增殖。结论转染Foxp3基因的Teff细胞具有抗移植排斥作用。

关键词: 植片排斥, 调节性T细胞, 高危, 穿透性角膜移植, Foxp3基因, 效应性T细胞

Abstract: Objective To observe the effects of Foxp3 gene-transfected Teff lymphocytes against the high-risk corneal transplantation rejection. Methods The Foxp3 gene-transfected Teff lymphocytes and the Teff lymphocytes were cultured together to observe the inhibitory effects on the Teff lymphocytes. Corneal neovascularization was induced by corneal suture in Balb/c mice and after one week, penetrating corneal transplantations were performed while Balb/c mice as receptors and C57/BL6 mice as donors. One day before the surgery, injection of Foxp 3 gene-transfected Teff lymphocytes were performed via tail vein of Balb/c mice. After the surgery, the state of corneal transplantation was monitored on a day to day basis and tail vein blood was sampled once every two weeks to check the lymphocyte types. Results The in vitro experiments showed strong inhibitory effects of Foxp3 gene-transfected Teff lymphocytes on the Teff lymphocytes. To the high-risk corneal transplantation mice, the Foxp3 gene-transfected Teff lymphocytes could moderately extend the survival time of the grafts (19.71±4.19d) while the survival time of the corneal grafts in the Treg group was 19.28±4.46d (P>0.05). On the contrary, the comparison between the Foxp3 gene-transfected Teff lymphocytes and the blank group (15.57± 2.22d) demonstrated some significance (P<0.05). Flow cytology indicated that the Foxp3 gene-transfected lymphocytes could delay the proliferation of CD4+T and CD8+T lymphocytes.Conclusion The Foxp3 gene-transfected Teff lymphocytes could prevent transplant rejection.

Key words: Teff, Treg, High risk, Foxp3 gene, Penetrate cornea transplant, Allograft rejection

中图分类号:

R779.6

赵军, 刘景, 王大江, 陈国玲, 王琪, 张晗. Foxp3基因转染的Teff细胞抗高危角膜移植排斥的作用[J]. 山东大学耳鼻喉眼学报, 2015, 29(1): 44-48.

ZHAO Jun, LIU Jing, WANG Da-jiang, CHEN Guo-ling, WANG Qi, ZHANG Han. Allograft rejection after high risk corneal transplantation with Foxp3 Gene-transferred effective T cells[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(1): 44-48.

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Foxp3基因转染的Teff细胞抗高危角膜移植排斥的作用

Allograft rejection after high risk corneal transplantation with Foxp3 Gene-transferred effective T cells

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