山东大学耳鼻喉眼学报 ›› 2021, Vol. 35 ›› Issue (5): 23-27.doi: 10.6040/j.issn.1673-3770.0.2020.536

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慢性鼻窦炎伴鼻息肉患者血清25-(OH)D3和组织中TGF-β1水平及临床意义

韩佳琦,苑国庆,朱宇彤,姜菲菲,阎艾慧   

  1. 中国医科大学附属第一医院 耳鼻咽喉科, 辽宁 沈阳 110001
  • 发布日期:2021-09-29
  • 通讯作者: 阎艾慧. E-mail:yah567@sina.com
  • 基金资助:
    辽宁省科技计划项目(20170058)

Serum 25-(OH)D3 and tissue TGF-β1 levels in patients with chronic rhino sinusitis with nasal polyps and their clinical significance

HAN Jiaqi, YUAN Guoqing, ZHU Yutong, JIANG Feifei, YAN Aihui   

  1. Department of Otolaryngology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China
  • Published:2021-09-29

摘要: 目的 探讨慢性鼻窦炎伴鼻息肉(CRSwNP)患者的血清25-(OH)D3水平和鼻息肉组织中转化生长因子-β1(TGF-β1)表达及相关性。 方法 收集住院手术并经病理诊断为慢性鼻窦炎伴鼻息肉患者47例为实验组,并以上颌窦囊肿患者21例为对照组。统计并比较两组患者血清25-(OH)D3水平和鼻息肉组织中TGF-β1平均光密度值,及两者之间相关性。 结果 实验组患者血清25-(OH)D3水平(16.97±7.35 μg/L)低于对照组患者(22.07±11.60 μg/L),差异有统计学意义(P=0.032);组织中TGF-β1平均光密度值(0.052 4±0.019 1)高于对照组患者(0.041 0±0.014 5),差异有统计学意义(P=0.018)。CRSwNP患者血清25-(OH)D3水平和鼻息肉组织中TGF-β1表达具有一定相关性(R2=0.204,∣R∣=0.452,P=0.019)。 结论 CRSwNP患者血清25-(OH)D3水平降低,而鼻息肉组织中TGF-β1表达升高,并且二者在CRSwNP中具有一定相关性。

关键词: 25-羟维生素D3, 转化生长因子-β1, 鼻息肉, 慢性鼻窦炎, 上颌窦囊肿, 光密度值

Abstract: Objective To investigate the relationship between serum 25-(OH)D3 levels and transforming growth factor-β1(TGF-β1)expression in patients with chronic rhinosinusitis with nasal polyps(CRSwNP). Methods Forty-seven patients with CRSwNP who were diagnosed by pathology were selected as an observation group and 21 patients with maxillary sinus cysts were selected as the control group. The level of serum 25-(OH)D3 and the mean optical density of TGF-β1 in the tissue samples were compared between the two factors. Results The level of serum 25-(OH)D3 in the observation group(16.97±7.35 μg/L)was lower than that in the control group(22.07±11.60 μg/L), the difference was statistically significant (P=0.032); the mean optical density of the TGF-β1 in the nasal polyps(0.052 4±0.019 1)was higher in the observation group than the control group(0.041 0±0.014 5); the difference was statistically significant(P=0.018). There was a significant correlation between serum 25-(OH)D3 levels and TGF-β1 expression in the nasal polyps(R2=0.204,|R|=0.452, P=0.019). Conclusion The level of serum 25-(OH)D3 in patients with CRSwNP decreased, while the expression of TGF-β1 in the nasal polyps increased, and there was a significant correlation between them in CRSwNP.

Key words: 25-(OH)D3, Transforming growth factor-β1, Chronic rhinosinusitis with nasal polyps, Chronic rhinosinusitis, Maxillary sinus cyst, Optical density value

中图分类号: 

  • R765.4
[1] Shi JB, Fu QL, Zhang H, et al. Epidemiology of chronic rhinosinusitis: results from a cross-sectional survey in seven Chinese cities[J]. Allergy, 2015, 70(5): 533-539. doi:10.1111/all.12577.
[2] Wierzbicka J, Piotrowska A, Zmijewski MA. The renaissance of vitamin D[J]. Acta Biochim Pol, 2014, 61(4): 679-686.
[3] Carroll WW, Schlosser RJ, O'Connell BP, et al. Vitamin D deficiency is associated with increased human sinonasal fibroblast proliferation in chronic rhinosinusitis with nasal polyps[J]. Int Forum Allergy Rhinol, 2016, 6(6): 605-610. doi:10.1002/alr.21704.
[4] Wang LF, Chien CY, Tai CF, et al. Vitamin D decreases the secretion of eotaxin and RANTES in nasal polyp fibroblasts derived from Taiwanese patients with chronic rhinosinusitis with nasal polyps[J]. Kaohsiung J Med Sci, 2015, 31(2): 63-69. doi:10.1016/j.kjms.2014.11.011.
[5] 张鹤, 张沄, 王宋平. 1, 25-二羟维生素D3通过TGF-β1/(Smad2/3)影响的ROS调节气道重塑[J]. 天津医药, 2020,48(4): 267-270,354. ZHANG He, ZHANG Yun, WANG Songping. Effects of 1,25-dihydroxyvitamin D3 on ROS regulating airway remodeling through TGF-β1/(Smad2/3)[J]. Tianjin Med J, 2020,48(4): 267-270,354.
[6] Hollis BW, Wagner CL. Clinical review: The role of the parent compound vitamin D with respect to metabolism and function: Why clinical dose intervals can affect clinical outcomes[J]. J Clin Endocrinol Metab, 2013, 98(12): 4619-4628. doi:10.1210/jc.2013-2653.
[7] Mostafa Bel-D, Taha MS, Abdel Hamid T, et al. Evaluation of vitamin D levels in allergic fungal sinusitis, chronic rhinosinusitis, and chronic rhinosinusitis with polyposis[J]. Int Forum Allergy Rhinol, 2016, 6(2): 185-190. doi:10.1002/alr.21585.
[8] Wang F, Yang Y, Chen H. Vitamin D deficiency are associated with subjective disease severity in Chinese patients with chronic rhinosinusitis with nasal polyps[J]. Am J Otolaryngol, 2019, 40(1): 36-39. doi:10.1016/j.amjoto.2018.09.003.
[9] 赵蓓蓓, 程雅婷, 董衡, 等. 25羟基维生素D检测方法的可靠性分析[J]. 临床儿科杂志, 2017, 35(12): 957-960. doi:10.3969/j.issn.1000-3606.2017.12.020.
[10] Adams JS, Hewison M. Update in vitamin D[J]. Clin Endocrinol Metab, 2010, 95(2): 471-478. doi: 10.1210/jc.2009-1773
[11] Bouillon R, Bischoff-Ferrari H, Willett W. Vitamin D and health: perspectives from mice and man[J]. J Bone Miner Res, 2008, 23(7): 974-979. doi: 10.1359/jbmr.080420
[12] Kamen DL, Tangpricha V. Vitamin D and molecular actions on the immune system: modulation of innate and autoimmunity[J]. J Mol Med(Berl), 2010, 88(5): 441-450. doi:10.1007/s00109-010-0590-9.
[13] Wjst M. Is vitamin D supplementation responsible for the allergy pandemic?[J]. Curr Opin Allergy Clin Immunol, 2012, 12(3): 257-262. doi:10.1097/ACI.0b013e3283535833.
[14] Bavi F, Movahed R, Salehi M, et al. Chronic rhinosinusitis with polyposis and serum vitamin D levels[J]. Acta Otorhinolaryngol Ital, 2019, 39(5): 336-340. doi:10.14639/0392-100X-2439.
[15] Rowan CJ, Li W, Martirosyan H, et al. Hedgehog-GLI signaling in Foxd1-positive stromal cells promotes murine nephrogenesis via TGFβ signaling[J]. Development, 2018, 145(13): dev159947. doi:10.1242/dev.159947.
[16] 刘争, 高起学, 张松, 等. 两种细胞外基质糖蛋白在鼻息肉中的表达[J]. 中华耳鼻咽喉科杂志, 2002, 37(3): 173-176. doi:10.3760/j.issn: 1673-0860.2002.03.005. LIU Zheng, GAO Qixue, ZHANG Song, et al. Expression of tenascin and fibronectin in nasal polyps[J]. Chin J Otorhinolaryngol, 2002, 37(3): 173-176. doi:10.3760/j.issn: 1673-0860.2002.03.005.
[17] 郭志娟, 李佩华, 张晓雯. 上皮间质转化状态在内镜治疗慢性鼻窦炎患者预后的预测意义研究[J]. 山东大学耳鼻喉眼学报, 2014,28(1): 34-38. doi:10.6040/j.issn.1673-3770.0.2013.243 GUO Zhijuan, LI Peihua, ZHANG Xiaowen. Prognostic significance of epithelial-mesenchymal transition state in patients with chronic sinusitis treated under endoscopy[J]. J Otolaryngol Ophthalmol Shandong Univ, 2014,28(1): 34-38. doi:10.6040/j.issn.1673-3770.0.2013.243.
[18] Fix C, Carver-Molina A, Chakrabarti M, et al. Effects of the isothiocyanate sulforaphane on TGF-β1-induced rat cardiac fibroblast activation and extracellular matrix interactions[J]. J Cell Physiol, 2019, 234(8): 13931-13941. doi:10.1002/jcp.28075.
[19] 矫健, 张罗. 转化生长因子-β1对慢性鼻-鼻窦炎伴鼻息肉上皮紧密连接屏障功能的影响[J]. 首都医科大学学报, 2018, 39(1): 45-50. doi:10.3969/j.issn.1006-7795.2018.01.009. JIAO Jian, ZHANG Luo. Effect of transforming growth factor-β1 on tight junction barrier function in nasal epithelial cells of chronic rhinosinusitis with nasal polyps[J]. J Cap Med Univ, 2018, 39(1): 45-50. doi:10.3969/j.issn.1006-7795.2018.01.009.
[20] Lee SA, Yang HW, Um JY, et al. Vitamin D attenuates myofibroblast differentiation and extracellular matrix accumulation in nasal polyp-derived fibroblasts through smad2/3 signaling pathway[J]. Sci Rep, 2017, 7(1): 7299. doi:10.1038/s41598-017-07561-6.
[21] Ramirez AM, Wongtrakool C, Welch T, et al. Vitamin D inhibition of pro-fibrotic effects of transforming growth factor beta1 in lung fibroblasts and epithelial cells[J]. J Steroid Biochem Mol Biol, 2010, 118(3): 142-150. doi:10.1016/j.jsbmb.2009.11.004.
[22] Shany S, Sigal-Batikoff I, Lamprecht S. Vitamin D and myofibroblasts in fibrosis and cancer: at cross-purposes with TGF-β/SMAD signaling[J]. Anticancer Res, 2016, 36(12): 6225-6234. doi:10.21873/anticanres.11216.
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