JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2016, Vol. 30 ›› Issue (1): 9-13.doi: 10.6040/j.issn.1673-3770.0.2015.490

Previous Articles     Next Articles

Catgut implantation at acupoints regulate immunity of sd rat with allergic rhinitis.

LIU Min1, ZHANG Dazheng1, LI Xinrong2, ZHANG Fubing3, WANG Xiaopei2, QI Yinhui4, ZHANG Qinxiu2.   

  1. Department of Otolaryngology, 1.Dujiangyan Peoples Hospital, Dujiangyan 611830, Sichuan, China;2.Teaching Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan, China;3.Leshan Hospital of Traditional Chinese Medicine, Leshan 614000, Sichuan, China;4.Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, Gansu, China
  • Received:2015-11-18 Online:2016-02-16 Published:2016-02-16

PDF (PC)

458

Abstract: Objective To explore the effect of catgut implantation at acupoints on allergic rhinitis(AR)animals and to discuss the mechanism based on pathological morphology and immunology. Methods A total of 70 healthy SPF SD rats were randomly divided into the blank group, the AR model group, the AR western medicine group, the AR false catgut implantation at acupoint group and the AR catgut implantation at acupoint group. AR animal models were established according to the method of improved ovalbumin injection and nasal mucosa stimulation. Every group received different therapies except the blank group. After the last treatment day, ovalbumin nasal mucosa stimulation was carried out, then the symptoms were observed and recorded. SD rats were killed and septum mucosa was collected to detect expressions of tissue TGF-β1 and IL-17 by using immunohistochemical method. Results Catgut implantation at acupoints can obviously alleviate the symptoms of sneezing, runny nose and scratching of AR rats. Compared with model group(Ⅱ), catgut implantation at acupoints can reduce the TGF-β1 and IL-17 content in the AR rat nasal mucosa. False catgut implantation at acupoints can reduce the TGF-β1 content. Conclusion Catgut implantation at acupoints can reduce the TGF-β 1 and IL-17 content to regulate immune system in AR rat nasal mucosa, then alleviate AR rats symptoms.

Key words: Animal experiment, Catgut implantation at acupoints, TGF-β1, IL-17, Allergic rhinitis, Immunity

CLC Number: 

  • R765.21
[1] 孔维佳.耳鼻咽喉头颈外科学[M].2版.北京:人民卫生出版社,2010:288.
[2] 李昕蓉,张勤修,刘敏,等.针灸治疗变应性鼻炎系统评价[J].辽宁中医药大学学报, 2013,15(3):51-57. LI Xinrong, ZHANG Qinxiu, LIU Min, et al. Acupuncture for allergic rhinitis: a systematic review[J]. J Liaoning Univ Trad Chin Med, 2013, 15(3):51-57.
[3] 刘敏,张大铮,李昕荣,等.卵白蛋白鼻腔强化激发制作变应性鼻炎大鼠模型效果观察[J].中国中西医结合耳鼻咽喉科杂志,2013,21(5):325-328. LIU Min, ZHANG Dazheng, LI Xinrong, et al. The effect of ovalbumin nasal strengthening stimulate for rat model of allergic rhinitis[J].Chin J Otorhinolaryngol Integ Med, 2013, 21(5):325-328.
[4] 华兴邦,李辞蓉,周浩良,等.大鼠穴位图谱的研制[J].实验动物与动物实验,1991,1(1):1-5.
[5] 黄桂锋.培土生金法治疗脾气虚变应性鼻炎大鼠的实验研究[D].成都:成都中医药大学,2010.
[6] 刘继辉.曲安奈德治疗变应性鼻炎68例[J].中国药业,2012,21(21):93-94.
[7] Park H, Li Z, Yang X O, et al. A distinct lineage of CD4+ T cells regulates tissue inflammation by Producing interleukin17[J].Natlmmunol,2005,6(11):1133-1141.
[8] 赵丽,马雄,邱德凯.Thl7细胞/调节性T细胞平衡与自身免疫性肝病[J].胃肠病学, 2009, 4(6):107-108.
[9] Korn T, Bettelli E, Gao W, et al. IL-17 and Thl7 Cells[J]. Arnu Rev lmmunol, 2009, 27:485- 517.
[10] Ouyang Y, Miyata M, Hatsushika K, et al. TGF-beta signaling may play a role in the development of goblet cell hyperplasia in a mouse model of allergic rhinitis[J]. Allergol Int, 2010, 59(3):313-319.
[11] 雷霏,许晶,徐洪君,等.实验性变应性鼻炎豚鼠鼻黏膜中TGF-β1表达及意义[J].中国老年学杂志,2008,28(6):533-534.
[12] Zhang H, Phan S. Inhibition of myofibroblast apoptosis by transforming growth factor-1[J]. Am J Respir Cell Mol Bio, 1999, 21(6):658-665.
[13] Lee S S, Won T B, Kim J W, et al. Effects of dexamethasone on the expression of transforming growth factor-[beta] in the mouse model of allergic rhinitis[J]. Laryngoscope, 2007, 117(8):1323-1328.
[14] 介华.曲安奈德鼻喷雾剂在过敏性鼻炎中的临床应用[J]. 中国实用医学,2012,7(21):183-184.
[15] 崔雪峰,田建华.曲安奈德鼻喷雾剂治疗变应性鼻炎的临床观察[J].承德医学院学报,2010,27(1):18-19.
[16] 谷小鸣,方薇,方润琪.曲安奈德鼻喷雾剂和伯克纳喷鼻剂治疗过敏性鼻炎的平行对照试验[J].中华临床医学杂志,2004,5(7):45-46.
[17] 李忠仁.实验针灸学[M]. 北京:中国中医药出版社,2003:329
[18] 刘争,陆翔,王恒.转化生长因子β1 白细胞介素-6及11和17在变应性鼻炎患者鼻黏膜中的表达[J].临床耳鼻咽喉科杂志, 2006, 20(14):625-627.
[1] WANG Tan, WU Ke, LI Lianqing, GONG Lili. Factors associated with systemic adverse reactions after subcutaneous immunotherapy injections and treatment options [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(5): 71-74.
[2] LU Hangui, LIN Xinsheng, YAO Danmian, WEI Yongxin, LI Chuangwei. Expression of IL-35 in allergic rhinitis and its effect on the immunoregulation of T cells [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(5): 66-70.
[3] PU Hongbo, DU Xiaodong. Allergen test results in 2 000 patients with allergic rhinitis in Wuxi District [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 105-107.
[4] CHEN Ming, YU Xuefei. Opinions on the treatment of chronic rhinosinusitis with allergic rhinitis [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(3): 18-22.
[5] WU Xiangping. Influence of patient management on the clinical effect of sublingual dermatophagoides farinae drops in patients [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(3): 68-72.
[6] LI Song, WANG Zonggui, YANG Jingpu, ZHANG Zhuping. Progress of transnasal endoscopic vidian neurectomy [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(1): 72-76.
[7] ZHI Lili, SONG Daoliang. Expressions of eosinophils and IL5 in antrochoanal polyps and nasal polyps. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(4): 43-46.
[8] WANG Hui, WEN Yan, YAN Li, YU Xiaoming, NING Hong. The effect of transforming growth factor-β1 on epithelial-mesenchymal transition in human lens epithelial cells. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(4): 76-79.
[9] WAN Wenjin, WANG Wen, CHENG Lei. A meta-analysis of subcutaneous immunotherapy and sublingual immunotherapy in the treatment of dust mite-allergic rhinitis. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(4): 103-108.
[10] LIU Huaitao, MA Ruixia, CHENG Lei. Surgical treatment of refractory allergic rhinitis. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(3): 18-21.
[11] GUAN Kai, LI Lisha. Nasal irrigation and its role in the treatment of allergic rhinitis. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(3): 22-27.
[12] ZHU Xinhua. Clinical applicationof rush immunotherapy for allergic rhinitis. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(3): 13-17.
[13] SHI Li, ZHAO Li, ZHANG Hongping. Long-term anti-inflammatory treatment of allergic rhinitis. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(3): 9-12.
[14] LIU Jing. Treatment of allergic rhinitis using syndrome differentiation based on the concept of “holism” in TCM. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(3): 28-30.
[15] CHENG Lei, QIAN Junjun, TIAN Huiqin. Research progresses on allergic rhinitis. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(3): 1-3.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd