Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2022, Vol. 36 ›› Issue (3): 254-259.doi: 10.6040/j.issn.1673-3770.0.2021.174

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Expression and significance of the MAPK,PI3K-AKT,NF-κB pathways of allergic rhinitis in mice

ZHANG Yaqi1, LIU Huimin1, CAO Linman1, WANG Ziyu1, LIN Xu1, LI Yanping2, XUE Gang2, WU Jingfang2   

  1. 1. School of Basic Medicine, Hebei North University, Zhangjiakou, 075700, Hebei, China;
    2. Key Laboratory for Accurate Diagnosis and Treatment of Thyroid Cancer, Zhangjiakou, 075700, Hebei, China
  • Published:2022-06-15

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Abstract: Objective To explore the mechanism of multi-signaling pathways proteins in the development of allergic rhinitis(AR)in mice. Methods Forty Kunming mice were randomly divided into allergic rhinitis group(AR group)and control group(Con group)each 20 mice. The nasal of 8 mice in each group was fixed with 4% paraformaldehyde, embedded in paraffin, sectioned and stained by HE method to observe the pathological changes of nasal mucosa. And the expression of MAPK, NF-κB and PI3K-AKT pathways proteins were detected by immunohistochemistry(IHC). The other 24 mice of each group were prepared for Western blot(WB)analysis. Results The behavioral results showed that scratching, sneezing and runny nose of the AR group were far more than Con group(P<0.05). In the HE staining, the mucosa showed pseudostratified ciliated columnar epithelial necrosis, epithelial structural remodeling and eosinophil infiltration etc. typical pathological changes of rhinitis. IHC showed that the positive proteins of three key pathways in the AR group was higher than that of the Con group. The proteins expression of MAPK, PI3K-AKT and NF-κB in the AR group were increased compared with the Con group and had a significant differences by western blot(P<0.05). Conclusion The increased proteins of MAPK, NF-κB and PI3K-AKT signaling pathways may have a synergistic effect on the pathogenesis of AR in mice.

Key words: Multiple signaling pathways, Allergic rhinitis, MAPK, AKT, NF-κB

CLC Number: 

  • R765.21
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