Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2021, Vol. 35 ›› Issue (4): 1-7.doi: 10.6040/j.issn.1673-3770.0.2020.399

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Mechanism of inhibition of ovalbumin-induced inflammation by catechins in an OVA-induced mouse model of allergic rhinitis

LI Huajing, HAO Runmei, DAI Hao, ZHANG Ling, SHEN Zhen, QUAN Fang, SHAO Yuan   

  1. Department of Otolaryngology & Head and Neck Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China
  • Published:2021-08-05

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Abstract: Objective This study aimed to analyze the mechanism of inhibition of ovalbumin(OVA)-induced inflammation by catechins in mice with allergic rhinitis. Methods BALB/c mice were randomly divided into a control group(OVA-induced mouse model of allergic rhinitis; model group); low-, medium-, and high-dose catechin groups(mice with allergic rhinitis treated with 50, 100, and 200 mg/kg of catechins, respectively), and a loratadine group(mice with allergic rhinitis treated with 2 mg/kg of loratadine). Each group included 10 mice. After treatment, the symptoms in each group were scored. Hematoxylin and eosin, Giemsa, periodic acid-Schiff, and toluidine blue were used to stain the nasal tissue sections. ELISA kits were used to detect Th1-related(TNF-α and IL-12), Th2-related(TNF-α, IL-1β, and IL-6), and Th17-related cytokine(IL-17A)levels in the mouse nasal lavage fluid(NALF). ELISA kits were also used to detect OVA-specific IgE and IgG1 in the mouse serum. Furthermore, we detected the protein expression of NF-κB p65, nucleus NF-κB p65, STAT3, p-STAT3, and ROR-γt in the mouse nasal mucosa using western blotting. Results The symptom scores of the middle- and high-dose catechin treatment groups were significantly lower than those of the model group(F=13.245, P<0.001). The catechin treatment groups had a lesser number of eosinophils, goblet cells, and mast cells in the nasal mucosa and lower levels of OVA-specific IgE and IgG1(F=15.462, P<0.001 and F=21.544, P<0.001, respectively)than the model group. The number of the abovementioned cells and levels OVA-specific IgE and IgG1 were reduced in a dose-dependent manner. In all groups, the levels of Th1-related cytokines in the NALF of mice did not significantly change(TNF-γ: F=0.753, P=0.600; IL-12: F=0.846, P=0.519).Furthermore, the catechin treatment groups had lower levels of Th2-related cytokines(TNF-α: F=11.100, P<0.001; IL-1β: F=12.554, P<0.001; IL-6: F=17.853, P<0.001), Th17-related cytokines(IL-17A: F=28.139, P<0.001), nucleus-NF-κB p65(F=17.573, P<0.001), ROR-γt(F=26.463, P<0.001), and p-STAT3(F=17.859, P<0.001)than the model group; there was a dose-dependent reduction in these levels. Conclusion Catechins have a good anti-inflammatory effect in mice with OVA-induced allergic rhinitis. The anti-inflammatory mechanism of catechins is related to correction of the Th1/Th2 cytokine balance, inhibition of the Th17 response, and inhibition of the NF-κB and ROR-γt/STAT3 pathways.

Key words: Allergic rhinitis, Catechins, Ovalbumin, Inflammation, Th1/Th2/Th17 cytokines

CLC Number: 

  • R765.21
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