关键词: 气管重建, 动物实验, 游离空肠, 异种(牛)脱细胞真皮基质, 抑制, 肠腺分泌

Abstract: Objective To study the construction of heterogeneous decellularized dermal matrix-free jejunal complex, in order to solve problem of intestinal secretion after the reconstruction of long-segment trachea with free jejunal graft. Methods A total of 10 adult mongrel dogs were involved. We cut the heterogeneous decellularized dermal matrix into suitable diameter, put it on the inner face of the jejunum whose mucous layer had been removed, and then used the Shape-memory Titanium-nickel Alloy stand to support it. On day 7, 14, 30, 90 and 180, we harvested the complex to observe the growth of epithelium with microscope. Results Of all 10 dogs, one dog died of intestinal adhesion and wound infection. The other 9 dogs lived to the end of the experiment. On day 7 and 14, we detected heterogeneous decellularized dermal matrix components, and part of free jejunal inner surface was squamous metaplasia. On day 30, 90 and 180, we did not detect any heterogeneous decellularized dermal matrix components, and almost all free jejunal inner surface was squamous metaplasia. Conclusion Heterogeneous decellularized dermal matrix with appropriate size could survive on the inner surface of the jejunum whose mucous layer had been removed and promote squamous metaplasia, thus inhibiting intestinal secretion. The Shape-memory Titanium-nickel Alloy stand could serve as a good support.

Key words: Intestinal secretion, Heterogeneous decellularized dermal matrix, Inhibition, Animal experiment, Free jejunum, Tracheal reconstruction