基于网络药理学探究小青龙汤治疗过敏性鼻炎的作用机制

doi:10.6040/j.issn.1673-3770.0.2021.134

摘要/Abstract

摘要： 目的基于网络药理学方法分析小青龙汤治疗过敏性鼻炎的作用机制,为临床运用及后期研究提供方向和参考。方法运用多个数据平台,分析小青龙汤对过敏性鼻炎的作用机制。在TCMSP数据库通过筛选OB、DL值,得到小青龙汤的有效化学成分及其作用靶点后使用Cytoscape3.7.1软件绘制小青龙汤有效成分-靶点网络关系图,并筛选出药物的关键成分。在Gencards、OMIM数据库获取过敏性鼻炎的潜在靶点。在GEO数据库获得有差异的基因,并将靶点纳入下一步的分析。通过在线画图软件,绘制韦恩图,提取小青龙汤作用靶点和过敏性鼻炎疾病靶点的交集靶点,而后对交集靶点进一步分析。在String平台对交集靶点进行蛋白相互作用分析,构建PPI网络,并将PPI网络关系导入Cytoscape3.7.1软件,筛选得到小青龙汤治疗过敏性鼻炎的核心靶点。采用Metascape平台分析小青龙汤-过敏性鼻炎交集靶点参与的生物过程及通路,得到其GO功能注释和KEGG通路富集分析数据后,使用OriginPro2020对其结果进行可视化分析,并运用Cytoscape3.7.1软件构建“小青龙汤-过敏性鼻炎交集靶点-信号通路”网络图。对关键成分和核心靶点用AutoDock软件进行分子对接验证。结果小青龙汤治疗过敏性鼻炎的关键化学成分为槲皮素、山柰酚、柚皮素等,核心靶点有STAT3、IL-6、TNF等。小青龙汤治疗过敏性鼻炎的信号通路主要作用于TNF信号通路、MAPK信号通路、PI3K-Akt信号通路、IL-17信号通路、缺氧诱导因子1信号通路等通路,其功能主要为受体配体活性、受体调节活性等。结论本研究初步揭示了小青龙汤治疗过敏性鼻炎的多成分、多靶点、多通路的作用机制,为小青龙汤的临床运用和研究提供参考。

关键词: 网络药理学, 分子对接, 过敏性鼻炎, 小青龙汤, 信号通路

Abstract: Objective To analyze the mechanism of action of the Xiaoqinglong decoction in allergic rhinitis treatment based on network pharmacology in order to provide direction and reference for its clinical application and further research. Methods Multiple data platforms were used to analyze the mechanism of action of the Xiaoqinglong decoction in allergic rhinitis. Through the screening of OB and DL values in the TCMSP database, the effective chemical components of the decoction, as well as their action targets, were obtained. The Cytoscape 3.7.1 software was used to draw the network diagram of the decoction's effective component-target and screening the drug's key components. Meanwhile, potential allergic rhinitis targets were obtained using the GenCards and OMIM databases, and the different genes were obtained from the GEO database and were included in the next analysis. Using an online drawing software, a Venn diagram was drawn to extract the intersection targets of the Xiaoqinglong decoction and allergic rhinitis disease targets for further analysis. Protein interaction analysis was then carried out for the intersection targets on the String platform for PPI network construction, which was imported into the Cytoscape3.7.1 software to screen the decoction core targets in allergic rhinitis treatment. The Metascape platform was used to analyze the biological processes and pathways involved in the intersection targets of Xiaoqinglong decoction-allergic rhinitis, and the GO and KEGG analyses data were obtained afterwards. The OriginPro2020 was then used to visualize the results obtained and analyzed using the Cytoscape 3.7.1 software, constructing the “Xiaoqinglong Decoction-allergic rhinitis intersection targets-signal pathway” diagram in the process. Additionally, AutoDock was used for molecular docking verification of these key components and core targets. Results The effective chemical components of the Xiaoqinglong decoction in allergic rhinitis treatment included quercetin, kaempferol, and naringenin, among others. Meanwhile, the identified core targets were STAT3, IL6, and TNF, among others. Moreover, the signaling pathway of the Xiaoqinglong decoction in allergic rhinitis treatment mainly acted on the TNF, MAPK, PI3K-Akt, IL-17, and HIF signaling pathways, wherein the molecular function was mainly found to mediate receptor ligand and receptor regulator activities, among others. Conclusion This study initially revealed the multi-component, multi-targeted, and multi-pathway mechanisms of action the Xiaoqinglong decoction in allergic rhinitis treatment, providing references for the drug's clinical applications.

Key words: Network pharmacology, Molecular docking, Allergic rhinitis, Xiaoqinglong Decoction

中图分类号:

R765.21

王鑫,刘巧平,闫占峰,刘思溟,朱雅静,丁倩,张莹,田媛,张京然. 基于网络药理学探究小青龙汤治疗过敏性鼻炎的作用机制[J]. 山东大学耳鼻喉眼学报, 2021, 35(5): 46-55.

WANG Xin, LIU Qiaoping, YAN Zhanfeng, LIU Siming, ZHU Yajing, DING Qian, ZHANG Ying, TIAN Yuan, ZHANG Jingran. A study on the mechanism of action of the Xiaoqinglong Decoction in treating allergic rhinitis based on network pharmacology[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(5): 46-55.

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