山东大学耳鼻喉眼学报 ›› 2021, Vol. 35 ›› Issue (4): 1-7.doi: 10.6040/j.issn.1673-3770.0.2020.399

• 论著 •    下一篇

儿茶素抑制卵清蛋白诱导的过敏性鼻炎小鼠模型炎症反应的机制研究

李化静,郝润梅,戴皓,张令,申震,权芳,邵渊   

  1. 西安交通大学第一附属医院 耳鼻咽喉头颈外科, 陕西 西安 710061
  • 发布日期:2021-08-05
  • 通讯作者: 邵渊. E-mail:Shaxiaoying1976@163.com
  • 基金资助:
    陕西省科技计划重点研发计划(2020-SF-A20);陕西省自然科学基础研计划(2020JM-382)

Mechanism of inhibition of ovalbumin-induced inflammation by catechins in an OVA-induced mouse model of allergic rhinitis

LI Huajing, HAO Runmei, DAI Hao, ZHANG Ling, SHEN Zhen, QUAN Fang, SHAO Yuan   

  1. Department of Otolaryngology & Head and Neck Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China
  • Published:2021-08-05

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摘要： 目的 研究儿茶素抑制卵清蛋白(OVA)诱导的过敏性鼻炎小鼠炎症反应的机制。 方法 将BALB/c小鼠随机分为对照组、模型组(OVA诱导的过敏性鼻炎小鼠模型),儿茶素低、中和高剂量组(用50、100和200 mg/kg的儿茶素治疗的过敏性鼻炎小鼠)和氯雷他定(2 mg/kg氯雷他定治疗的过敏性鼻炎小鼠)组,每组10只。治疗后对各组小鼠进行症状评分。使用苏木精-伊红、Giemsa、Periodic acid-Schiff和甲苯胺蓝对鼻组织切片进行染色。使用ELISA试剂盒检测小鼠鼻腔灌洗液(NALF)中的Th1相关细胞因子(TNF-γ和IL-12)、Th2相关细胞因子(TNF-α、IL-1β和IL-6)、Th17相关细胞因子(IL-17A)水平。使用ELISA试剂盒检测小鼠血清中OVA特异性IgE和IgG1。通过Western blot检测小鼠鼻黏膜组织中NF-κB p65、nucleus-NF-κB p65、STAT3、p-STAT3和ROR-γt的蛋白表达。 结果 与模型组相比,儿茶素中剂量和高剂量组小鼠的症状评分明显降低(F=13.245,P<0.001);儿茶素治疗以剂量依赖性方式降低了鼻黏膜组织中的嗜酸性粒细胞、杯状细胞和肥大细胞数(F=15.462,P<0.001);儿茶素治疗以剂量依赖性方式降低了小鼠血清OVA特异性IgE和IgG1的水平(F=21.544,P<0.001)。各组小鼠NALF中Th1相关细胞因子水平无变化(TNF-γ:F=0.753,P=0.600;IL-12:F=0.846,P=0.519)。与模型组相比,儿茶素治疗以剂量依赖性方式降低了小鼠NALF中Th2(TNF-α:F=11.100,P<0.001;IL-1β:F=12.554,P<0.001;IL-6:F=17.853,P<0.001)和Th17相关细胞因子的水平(IL-17A:F=28.139,P<0.001)。与模型组相比,儿茶素治疗以剂量依赖性方式降低了小鼠鼻黏膜组织中nucleus-NF-κB p65(F=17.573,P<0.001)、ROR-γt(F=26.463,P<0.001)和p-STAT3的水平(F=17.859,P<0.001)。 结论 儿茶素在OVA诱导的过敏性鼻炎小鼠模型中具有较好的抗炎效果。儿茶素的抗炎机制与纠正Th1/Th2细胞因子平衡、抑制Th17反应、抑制NF-κB和ROR-γt/ STAT3途径有关。

关键词: 过敏性鼻炎, 儿茶素, 卵清蛋白, 炎症, Th1/Th2/Th17细胞因子

Abstract: Objective This study aimed to analyze the mechanism of inhibition of ovalbumin(OVA)-induced inflammation by catechins in mice with allergic rhinitis. Methods BALB/c mice were randomly divided into a control group(OVA-induced mouse model of allergic rhinitis; model group); low-, medium-, and high-dose catechin groups(mice with allergic rhinitis treated with 50, 100, and 200 mg/kg of catechins, respectively), and a loratadine group(mice with allergic rhinitis treated with 2 mg/kg of loratadine). Each group included 10 mice. After treatment, the symptoms in each group were scored. Hematoxylin and eosin, Giemsa, periodic acid-Schiff, and toluidine blue were used to stain the nasal tissue sections. ELISA kits were used to detect Th1-related(TNF-α and IL-12), Th2-related(TNF-α, IL-1β, and IL-6), and Th17-related cytokine(IL-17A)levels in the mouse nasal lavage fluid(NALF). ELISA kits were also used to detect OVA-specific IgE and IgG1 in the mouse serum. Furthermore, we detected the protein expression of NF-κB p65, nucleus NF-κB p65, STAT3, p-STAT3, and ROR-γt in the mouse nasal mucosa using western blotting. Results The symptom scores of the middle- and high-dose catechin treatment groups were significantly lower than those of the model group(F=13.245, P<0.001). The catechin treatment groups had a lesser number of eosinophils, goblet cells, and mast cells in the nasal mucosa and lower levels of OVA-specific IgE and IgG1(F=15.462, P<0.001 and F=21.544, P<0.001, respectively)than the model group. The number of the abovementioned cells and levels OVA-specific IgE and IgG1 were reduced in a dose-dependent manner. In all groups, the levels of Th1-related cytokines in the NALF of mice did not significantly change(TNF-γ: F=0.753, P=0.600; IL-12: F=0.846, P=0.519).Furthermore, the catechin treatment groups had lower levels of Th2-related cytokines(TNF-α: F=11.100, P<0.001; IL-1β: F=12.554, P<0.001; IL-6: F=17.853, P<0.001), Th17-related cytokines(IL-17A: F=28.139, P<0.001), nucleus-NF-κB p65(F=17.573, P<0.001), ROR-γt(F=26.463, P<0.001), and p-STAT3(F=17.859, P<0.001)than the model group; there was a dose-dependent reduction in these levels. Conclusion Catechins have a good anti-inflammatory effect in mice with OVA-induced allergic rhinitis. The anti-inflammatory mechanism of catechins is related to correction of the Th1/Th2 cytokine balance, inhibition of the Th17 response, and inhibition of the NF-κB and ROR-γt/STAT3 pathways.

Key words: Allergic rhinitis, Catechins, Ovalbumin, Inflammation, Th1/Th2/Th17 cytokines

中图分类号: 

  • R765.21
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