ERK5蛋白在鼻息肉细胞中的表达和意义

doi:10.6040/j.issn.1673-3770.0.2015.310

摘要/Abstract

摘要： 目的探讨细胞外调节蛋白激酶5(ERK5)在鼻息肉细胞中的表达和意义。方法分析2013年1月至2014年12月接受治疗的慢性鼻-鼻窦炎(CRS)患者的临床资料,将其中37例慢性鼻-鼻窦炎伴鼻息肉(CRSwNP)患者列为观察组(CRSwNP组),35例慢性鼻-鼻窦炎不伴鼻息肉(CRSsNP)患者作为对照组(CRSsNP组)。结果 CRSwNP组患者血清中TNF-α(t=16.7,P<0.001)、IL-5(t=13.81,P<0.001)和IL-8(t=8.642,P<0.001)含量显著高于对照组,差异具有统计学意义(P>0.05);Western Boltting检测结果表明,在CRSwNP组中ERK5蛋白表达水平显著高于对照组中ERK5蛋白表达水平(灰度比1.21±0.16 vs 0.36±0.04,P<0.01,n=37);Pearson相关性检验分析结果表明,CRSwNP组患者ERK5蛋白表达与TNF-α(r=0.521,P=0.001)及IL-8(r=0.479,P=0.003)呈显著正相关。结论 ERK5蛋白在CRSwNP患者中高水平表达,且与TNF-α及IL-8呈显著正相关关系。

关键词: 细胞外调节蛋白激酶5, 酶联免疫吸附试验, 鼻息肉

Abstract: Objective To investigate the expression and significance of extracellular regulated protein kinases 5(ERK5)protein in nasal polyps cells. Methods The clinical data of patients with chronic rhinosinusitis in our hospital from January 2013 to December 2014 was analyzed, in which 37 patients with nasal polyps were treated as the observation group(CRSwNP group)and 35 patients without nasal polyps were treated as the control group(CRSsNP group). Results Serum TNF-α(t=16.7, P<16.7), IL-5 (t=13.81, P<0.001)and IL-8(t=8.642, P<0.001)levels of the patients in the CRSwNP group were significantly higher than those in the control group, the difference had statistical significance(P>0.05). The results of western boltting test found that ERK5 protein expression level in the CRSwNP group was significantly higher than in the control group(gamma ratio 1.21±0.16 vs 0.36±0.04, P<0.01, n=37). By using Pearson correlation test ERK5 protein expression of the CRSwNP group had significant positive correlation with TNF-α(r=0.521, P=0.001)and IL-8(r=0.479, P=0.479)level. Conclusion ERK5 protein in patients with nasal polyps show high expression level and positive correlation with TNF-α and IL-8 level.

Key words: Nasal polyp, Extracellular regulated protein kinases 5, ELISA

中图分类号:

R765.2

高振峰,陶谦,蔡晓岚. ERK5蛋白在鼻息肉细胞中的表达和意义[J]. 山东大学耳鼻喉眼学报, 2016, 30(1): 40-42.

GAO Zhenfeng, TAO Qian, CAI Xiaolan. Expression and significance of ERK5 protein in nasal polyps cells.[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(1): 40-42.

参考文献

[1] Miranda M, Rozali E, Khanna K K, et al. MEK5-ERK5 pathway associates with poor survival of breast cancer patients after systemic treatments[J]. Oncoscience, 2015, 2(2):99-101.
[2] Finegan K G, Perez-Madrigal D, Hitchin J R, et al. ERK5 is a critical mediator of inflammation-driven cancer[J]. Cancer Res, 2015, 75(4):742-753.
[3] 尹宏, 黄欣, 王海燕, 等. ERK5在胃腺癌组织中的表达及临床意义[J]. 中国现代医学杂志, 2014, 24(20):56-60. YIN Hong, HUANG Xin, WANG Haiyan, et al. Expression of ERK5 in gastric adenocarcinoma and its clinical significance[J]. Chin J Mod Med, 2014, 24(20):56-60.
[4] Yue B, Ren Q X, Su T, et al. ERK5 silencing inhibits invasion of human osteosarcoma cell via modulating the Slug/MMP-9 pathway[J]. Eur Rev Med Pharmacol Sci, 2014, 18(18):2640-2647.
[5] 中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组, 中华医学会耳鼻咽喉头颈外科学分会鼻科学组. 慢性鼻-鼻窦炎诊断和治疗指南(2012, 昆明)[J]. 中华耳鼻咽喉头颈外科杂志, 2013, 48(2):92-94.
[6] 王耀晟, 程晓曙, 洪葵, 等. 细胞外信号调节蛋白激酶5/凋亡调节蛋白信号途径在低温诱导心肌细胞损伤及凋亡中的调控作用[J]. 中国循环杂志, 2014(7):540-544. WANG Yaosheng, CHENG Xiaoshu, HONG Kui, et al. The regulative role of ERK5/Bim pathway in hypothermal stimulation induced neonatal rats cardiomyocytes injury and apoptosis[J]. Chin Circ J, 2014(7):540-544.
[7] Xiu Q, Meng C, Li L, et al. Allergic factors affect on severity of chronic rhinosinusitis and postoperative outcomes[J]. Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi, 2015, 29(1):34-38, 44.
[8] Koseoglu S, Ozcan K M, Ikinciogullari A, et al. Evaluation of autoinflammatory disease genes in nasal polyposis[J]. Turk J Med Sci, 2015, 45(1):136-140.
[9] Wang C, Lou H, Wang X, et al. Effect of budesonide transnasal nebulization in patients with eosinophilic chronic rhinosinusitis with nasal polyps[J]. J Allergy Clin Immunol, 2015, 135(4):922-929.e6.
[10] Poposki J A, Peterson S, Welch K, et al. Elevated presence of myeloid dendritic cells in nasal polyps of patients with chronic rhinosinusitis[J]. Clin Exp Allergy, 2015, 45(2):384-393.
[11] Heo K S, Cushman H J, Akaike M, et al. ERK5 activation in macrophages promotes efferocytosis and inhibits atherosclerosis[J]. Circulation, 2014, 130(2):180-191.

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