山东大学耳鼻喉眼学报 ›› 2025, Vol. 39 ›› Issue (1): 61-67.doi: 10.6040/j.issn.1673-3770.0.2024.027

• 论著 • 上一篇    

藏红花素在嗜酸性慢性鼻窦炎伴鼻息肉中调控2型炎症反应的研究

张婕1,2,尼玛吉宗2,徐小东3,周菁4,刘建敏4,罗依蕤1,2,杜进涛4,巴罗1,2   

  1. 1.西藏大学医学院, 西藏 拉萨 850000 ;
    2.西藏自治区人民医院 耳鼻咽喉科, 西藏 拉萨 850000 ;
    3.山南市人民医院 耳鼻喉科, 西藏 山南 856000;
    4.四川大学华西医院 耳鼻咽喉头颈外科, 四川 成都 610000
  • 发布日期:2025-01-17
  • 通讯作者: 巴罗. E-mail:bhanor@163.com

Effect of Crocin on Type 2 inflammation in eosinophilic chronic rhinosinusitis with nasal polyps

ZHANG Jie1,2, NIMA Jizong2, XU Xiaodong3, ZHOU Jing4, LIU Jianmin4, LUO Yirui1,2, DU Jintao4, BA Luo1,2   

  1. 1. Medical College of Tibet University, Lasa 850000, Xizang, China 2. Department of Otorhinolaryngology, People's Hospital of Tibet Autonomous Region, Lasa 850000, Xizang, China3. Department of Otolaryngology, Shannan City People's Hospital, Shannan 856000, Xizang, China4. Department of Otorhinolaryngology & Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan,  China
  • Published:2025-01-17

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摘要： 目的 探讨藏红花素在嗜酸性粒细胞性慢性鼻窦炎伴鼻息肉(eosinophilic chronic rhinosinusitis with nasal polyps, Eos CRSwNP)患者外周血单个核细胞(peripheral blood mononuclear cell, PBMC)2型炎症反应中的调控作用和作用机制,为精准治疗鼻息肉提供新的靶点或通路。 方法 收集慢性鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps, CRSwNP)患者鼻息肉组织进行病理学诊断,根据炎症细胞类型分为嗜酸性粒细胞性慢性鼻窦炎伴鼻息肉(eosinophilic chronic rhinosinusitis with nasal polyps, Eos CRSwNP)和非嗜酸性粒细胞性慢性鼻窦炎伴鼻息肉(non-eosinophilic chronic rhinosinusitis with nasal polyps, Non-Eos CRSwNP)。密度梯度离心法提取Eos CRSwNP患者外周血中的PBMC。用不同浓度IL-33刺激PBMC,qPCR检测转录因子GATA3、T-bet和细胞受体CRTH2,qPCR和ELISA分析炎症细胞因子INF-γ,IL-5和IL-13的表达情况。再加入不同浓度藏红花素进行干预,Western blotting和qPCR检测GATA3、T-bet和CRTH2。Graphpad prism软件进行统计分析,对比IL-33刺激前后、藏红花素干预前后指标变化有无显著差异。 结果 共有16例经病理学确诊为Eos CRSwNP。不同浓度的IL-33刺激PBMC后,qPCR证实T-bet,GATA3和CRTH2的表达有明显升高(P值分别为0.004、0.001、0.026);qPCR和ELISA进一步发现INF-γ,IL-5和IL-13等细胞因子上调(qPCR的P值分别为0.001、0.001、0.003;ELISA的P值分别为0.035、0.006、0.001),其中IL-33浓度为20 ng/mL时对PBMC炎症细胞活化最为明显。再加入藏红花素干预后,Western blotting和qPCR检测显示T-bet,GATA3和CRTH2表达呈下降趋势,且在浓度为10 ug/mL 时最为明显(WB的P值分别为0.027、0.001、0.002;qPCR的P值分别为0.019、0.001、0.001)。 结论 藏红花素能够显著抑制IL-33激活的CRSwNP患者内的2型炎症反应,具有潜在的CRSwNP新型治疗药物开发价值。

关键词: 慢性鼻窦炎, 鼻息肉, 嗜酸性粒细胞, 外周血单个核细胞, 藏红花素, 2型炎症反应

Abstract: Objective To study the effect of crocin on Eosinophilic Chronic Rhinosinusitis with Nasal Polyps, the regulatory role and mechanism of Peripheral blood mononuclear cell(PBMC)type 2 inflammation in patients with Eos CRSwNP, providing a new target or pathway for precise treatment of nasal polyps. Methods Chronic rhinosinusitis with nasal polyps(CRSwNP)patients were collected for pathological diagnosis. Eosinophilic Chronic Rhinosinusitis with Nasal Polyps(Eos CRSwNP)and Non-eosinophilic Chronic Rhinosinusitis with Nasal Polyps(Non-Eos CRSwNP)were classified according to the inflammatory cell types. PBMC in peripheral blood of Eos CRSwNP patients was extracted by density gradient centrifugation. PBMC was stimulated with different concentrations of IL-33, and transcription factors GATA3, T-bet, and cell receptor CRTH2 were detected by qPCR. The expression of inflammatory cytokines INF-γ, IL-5, and IL-13 was analyzed by qPCR and ELISA. Then different concentrations of crocin were added for intervention, and GATA3, T-bet, and CRTH2 were detected by Western blotting and qPCR. GraphPad Prism software was used for statistical analysis to compare whether there were significant differences in index changes before and after IL-33 stimulation and crocin intervention. Results A total of 16 cases were pathologically confirmed as Eos CRSwNP. After stimulating PBMC with different concentrations of IL-33, qPCR confirmed that the expression of T-bet, GATA3, and CRTH2 was significantly increased(P values were 0.004, 0.001, and 0.026, respectively). qPCR and ELISA further revealed upregulation of cytokines INF-γ, IL-5, and IL-13(P values of qPCR were 0.001, 0.001, and 0.003, respectively; P values of ELISA were 0.035, 0.006, and 0.001, respectively), with the most significant activation of PBMC inflammatory cells observed at an IL-33 concentration of 20 ng/mL. After intervention with crocin, Western blotting and qPCR showed a downward trend in the expression of T-bet, GATA3, and CRTH2, with the most significant effect observed at a concentration of 10 μg/mL(P values of WB were 0.027, 0.001, and 0.002, respectively; P values of qPCR were 0.019, 0.001, and 0.001, respectively). Conclusion Crocin can significantly inhibit type 2 inflammation in IL-33-activated CRSwNP patients and has potential value in the development of new therapeutic drugs for CRSwNP.

Key words: Chronic rhinosinusitis, Nasal polyps, Eosinophils, Peripheral blood mononuclear cells, Crocin, Type 2 inflammatory effect

中图分类号: 

  • R765.41
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