糖尿病视网膜病变患者血清Sestrin2和NADPH氧化酶2水平与眼底病变的相关性

doi:10.6040/j.issn.1673-3770.0.2024.068

摘要/Abstract

摘要： 目的探讨糖尿病视网膜病变(diabetic retinopathy, DR)患者血清应激诱导蛋白2(Sestrin2)和NADPH氧化酶2(NOX2)水平与眼底病变的相关性。方法对到我院眼科门诊就诊的139例糖尿病患者,根据患者的眼科临床诊断结果分为DR组和非DR组,根据DR患者病情分期将DR组分为增生型DR组和非增生型DR组,分析患者临床资料及入院时血清中Sestrin2和NOX2的表达水平。采用Pearson 分析眼底病变严重程度与血清Sestrin2和NOX2表达量的相关性,通过多因素Logistic回归模型分析患DR的影响因素。结果 DR组血清中Sestrin2水平［(1.97±0.38)ng/mL］低于非DR组［(3.74±0.84)ng/mL］,而NOX2水平［(11.38±1.59)ng/mL］高于非DR组［(6.75±1.69)ng/mL］(P<0.05)。血清Sestrin2在非增生型DR组的表达量显著高于增生型DR组,且随着病情的加重而降低(P<0.05),NOX2在非增生型DR组表达量显著低于增生型DR组,且随着病情的加重而升高(P<0.05)。Logistic回归分析显示,高INS含量和高Sestrin2水平是DR的保护因素,血清中高空腹血糖(fasting plasma glucose, FPG)及高NOX2是DR的危险因素(P<0.05)。结论 DR患者血清中Sestrin2呈现低水平,NOX2的表达水平则提高,且二者的变化水平与DR患者眼底病变关系密切。

关键词: 糖尿病, 视网膜病变, Sestrin2, NADPH氧化酶2, 氧化应激

Abstract: Objective To explore the correlation between serum levels of stress-inducing protein 2(Sestrin2)and NADPH oxidase 2(NOX2)and fundus lesions in patients with diabetic retinopathy(DR). Methods 139 patients with diabetes who visited our ophthalmic clinic. According to the clinical diagnosis of the patient's ophthalmology, the patients were grouped into the DR group and the non-DR group, and then according to the stage of the disease of the DR patients, the DR group was divided into the hyperplastic DR group and the non-hyperplastic DR group. Clinical data and serum Sestrin2 and NOX2 expression levels were analyzed. Pearson's correlation between the severity of fundus lesions and serum Sestrin2 and NOX2 expression levels was analyzed by Pearson; Multivariate logistic regression model was applied to analyze the factors influencing DR. Results The serum Sestrin2 content in the DR group ［(1.97±0.38)ng/mL］ was obviously lower than in the non-DR group ［(3.74±0.84)ng/mL), while the NOX2 content ［(11.38±1.59)ng/mL］ was much higher than that in the non-DR group ［(6.75±1.69)ng/mL］(P<0.05). The expression level of serum Sestrin2 in the non-proliferative DR group was obviously higher than in the proliferative DR group and decreased with the aggravation of the disease(P<0.05). The level of NOX2 expression in the non-proliferative DR group was obviously lower than in the proliferative DR group and increased with the aggravation of the disease(P<0.05). Logistic regression analysis showed that high levels of Sestrin2 were a protective factor for DR, while high serum FPG and high NOX2 were risk factors for DR(P<0.05). Conclusion The expression level of Sestrin2 is low, the expression level of NOX2 is increased, and its changes are closely related to fundus lesions in patients with DR.

Key words: Diabetes, Retinopathy, Sestrin2, NADPH oxidase 2, Oxidative stress

中图分类号:

R774.1

高琳,何金梅,杨明祎. 糖尿病视网膜病变患者血清Sestrin2和NADPH氧化酶2水平与眼底病变的相关性[J]. 山东大学耳鼻喉眼学报, 2025, 39(6): 113-117.

GAO Lin, HE Jinmei, YANG Mingyi. Correlation between serum levels of Sestrin2 and NADPH oxidase-2 and fundus lesions in patients with diabetes retinopathy[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 113-117.

参考文献

[1] Lu HN, Yang JY, Li J, et al. MiR-190 ameliorates glucotoxicity-induced dysfunction and apoptosis of pancreatic β-cells by inhibiting NOX2-mediated reactive oxygen species production[J]. PeerJ, 2022, 10: e13849. doi:10.7717/peerj.13849
[2] Mendonca HR, Carpi-Santos R, da Costa Calaza K, et al. Neuroinflammation and oxidative stress act in concert to promote neurodegeneration in the diabetic retina and optic nerve: galectin-3 participation[J]. Neural Regen Res, 2020, 15(4): 625-635. doi:10.4103/1673-5374.266910
[3] Kowluru RA. Diabetic retinopathy and NADPH oxidase-2: a sweet slippery road[J]. Antioxidants(Basel), 2021, 10(5): 783. doi:10.3390/antiox10050783
[4] 边云, 白海龙, 孟晓峰, 等. 贞莲明目胶囊治疗糖尿病视网膜病变的网络药理学机制分析[J]. 实用临床医药杂志, 2023, 27(18): 75-82. doi:10.7619/jcmp.20232153 BIAN Yun, BAI Hailong, MENG Xiaofeng, et al. Analysis of network pharmacological mechanism of Zhenlian Mingmu Capsule in treatment of diabetic retinopathy[J]. Journal of Clinical Medicine in Practice, 2023, 27(18): 75-82. doi:10.7619/jcmp.20232153
[5] Tonade D, Kern TS. Photoreceptor cells and RPE contribute to the development of diabetic retinopathy[J]. Prog Retin Eye Res, 2021, 83: 100919. doi:10.1016/j.preteyeres.2020.100919
[6] Yang XL, Li DL. Tricin attenuates diabetic retinopathy by inhibiting oxidative stress and angiogenesis through regulating Sestrin2/Nrf2 signaling[J]. Hum Exp Toxicol, 2023, 42: 9603271231171642. doi:10.1177/09603271231171642
[7] 中华医学会糖尿病学分会. 中国2型糖尿病防治指南(2017年版)[J]. 中国实用内科杂志, 2018, 38(4): 292-344. doi:10.19538/j.nk2018040108 Chinese Diabetes Society. Guidelines for the prevention and control of type 2 diabetes in China(2017 edition)[J]. Chinese Journal of Practical Internal Medicine, 2018, 38(4): 292-344. doi:10.19538/j.nk2018040108
[8] 中华医学会眼科学会眼底病学组. 我国糖尿病视网膜病变临床诊疗指南(2014年)[J]. 中华眼科杂志, 2014, 50(11): 851-865. doi:10.3760/cma.j.issn.0412-4081.2014.11.014
[9] 王娇娇, 李苗, 宋宗明. 糖尿病视网膜病变的机制和细胞模型研究进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(5): 93-99. doi:10.6040/j.issn.1673-3770.0.2021.203 WANG Jiaojiao, LI Miao, SONG Zongming. Progress in diabetic retinopathy mechanisms and cellular models[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(5): 93-99. doi:10.6040/j.issn.1673-3770.0.2021.203
[10] Takele MB, Boneya DJ, Alemu HA, et al. Retinopathy among adult diabetics and its predictors in northwest Ethiopia[J]. J Diabetes Res, 2022, 2022: 1362144. doi:10.1155/2022/1362144
[11] Su ZY, Liu W, Yang JK. Association between proliferative diabetic retinopathy and serum bile acid level in patients with type 2 diabetes mellitus[J]. Endocr Metab Immune Disord Drug Targets, 2021, 21(11): 2063-2067. doi:10.2174/1871530321666210112160724
[12] Korhonen A, Gucciardo E, Lehti K, et al. Proliferative diabetic retinopathy transcriptomes reveal angiogenesis, anti-angiogenic therapy escape mechanisms, fibrosis and lymphatic involvement[J]. Sci Rep, 2021, 11(1): 18810. doi:10.1038/s41598-021-97970-5
[13] Yang XL, Wu XL. The impact of sestrin2 on reactive oxygen species in diabetic retinopathy[J]. Cell Biochem Funct, 2024, 42(4): e4024. doi:10.1002/cbf.4024
[14] Jiang Y, Luo B. Histone deacetylase 3 inhibitor attenuates diabetic retinopathy in mice[J]. J Neurophysiol, 2023, 129(1): 177-183. doi:10.1152/jn.00477.2022
[15] Alka K, Mohammad G, Kowluru RA. Regulation of serine palmitoyl-transferase and Rac1-Nox2 signaling in diabetic retinopathy[J]. Sci Rep, 2022, 12(1): 16740. doi:10.1038/s41598-022-20243-2
[16] 刘谦, 周健, 武珅, 等. NOX2基因缺陷对rd1小鼠感光细胞凋亡的保护作用[J]. 眼科, 2022, 31(2): 140-145. doi:10.13281/j.cnki.issn.1004-4469.2022.02.012 LIU Qian, ZHOU Jian, WU Shen, et al. Protection of photoreceptor degeneration in NADPH oxidase 2 deficiency-rd1 mice[J]. Ophthalmology in China, 2022, 31(2): 140-145. doi:10.13281/j.cnki.issn.1004-4469.2022.02.012

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