咽喉反流患者唾液菌群与反流症状相关性分析

doi:10.6040/j.issn.1673-3770.0.2024.111

摘要/Abstract

摘要： 目的研究咽喉反流(laryngopharyngeal reflux, LPR)患者唾液菌群与反流症状的相关性。方法选取2022年2月至2022年11月就诊于解放军总医院第八医学中心耳鼻咽喉头颈外科门诊反流症状指数(reflux symptom index, RSI)评分>13分、质子泵抑制剂诊断性治疗显效的34例患者为LPR组, 34例健康志愿者为对照组,留取唾液标本,提取 DNA,扩增 16S 片段V3-V4区并进行测序,对测序结果进行生物信息学分析,对LEfSe分析的差异菌与RSI评分中9个症状评分及总分的相关性进行分析,使用 SPSS 25.0 软件对数据进行统计学处理。结果 LEfSe分析显示鼠乳杆菌、乳酸杆菌科、乳酸杆菌属、普雷沃菌科、普雷沃菌属、拟杆菌目、拟杆菌纲、拟杆菌门等30个物种在LPR组患者唾液中富集,厚壁菌门、杆菌纲、假单胞菌属等28个物种在对照组唾液中富集。相关分析显示拟杆菌门、拟杆菌纲、拟杆菌目、普雷沃菌科、普雷沃菌属相对丰度与胃烧灼感、胸痛、消化不良或胃疼症状评分呈现正相关,普雷沃菌属的相对丰度与该症状的相关系数具有统计学意义(P<0.05),口腔普雷沃菌相对丰度与吞咽食物、液体和药片困难呈正相关(P<0.05),乳酸杆菌科、乳酸杆菌属相对丰度与RSI 总分呈负相关(P<0.05)。结论 LPR患者唾液菌群失调与反流症状相关,调整菌群失调可能有助于减轻患者症状。

关键词: 咽喉反流, 唾液菌群, 反流症状指数, 相关性分析

Abstract: Objective To investigate the correlation between salivary microbiota and symptoms in laryngopharyngeal reflux(LPR)patients. Methods Patients with a reflux symptom index(RSI)score>13 and an apparent response after 8 weeks of proton pump inhibitor medication were enrolled as the LPR group in the Department of Otorhinolaryngology Head and Neck Surgery, the Eighth Medical Center, Chinese PLA General Hospital from February 2022 to November 2022. Another 34 matched healthy volunteers were enrolled as the control group. Their saliva samples were collected, and salivary microbiota was detected and analysed by 16S rRNA gene sequencing. The correlation between differential bacteria in LEfSe analysis and RSI scores was investigated. SPSS 25.0 software was used for statistical analysis. Results LEfSe difference analysis showed that Lactobacillus_murinus, Lactobacillaceae, Lactobacillus, Prevotellaceae, Prevotella, Bacteroidales, Bacteroidia, Bacteroidetes and other 30 species were enriched in the LPR group. Firmicutes, Bacilli, Pseudomonas and other 28 species were enriched in the control group. The correlation analysis demonstrated that the relative abundance of Bacteroidetes, Bacteroidetes, Bacteroidetes, Prevotellaceae and Prevotella were positively correlated with the symptom score of gastric burning sensation, chest pain, dyspepsia or stomachache, and the relative abundance of Prevotella was significantly correlated with the above symptoms(P<0.05). The relative abundance of Prevotella_oralis was positively correlated with the symptom score of difficulty to swallow food, liquids, and tablets(P<0.05). The relative abundance of Lactobacillaceae and Lactobacillu was negatively related to the total score of RSI. Conclusion The salivary dysbiosis might be related to the reflux symptoms of LPR patients and modify the imbalance of ecology in saliva might help to alleviate the symptoms.

Key words: Laryngopharyngeal reflux, Salivary microbiota, Reflux symptom index, Correlation analysis

中图分类号:

R766.5

崔小缓,尹龙龙,张延平,蒋兴旺,李丽娜. 咽喉反流患者唾液菌群与反流症状相关性分析[J]. 山东大学耳鼻喉眼学报, 2024, 38(6): 78-84.

CUI Xiaohuan, YIN Longlong, ZHANG Yanping, JIANG Xingwang, LI Lina. Study of the association between salivary microbiota and reflux symptoms in patients with laryngopharyngeal reflux[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(6): 78-84.

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