Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2021, Vol. 35 ›› Issue (5): 46-55.doi: 10.6040/j.issn.1673-3770.0.2021.134

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A study on the mechanism of action of the Xiaoqinglong Decoction in treating allergic rhinitis based on network pharmacology

WANG Xin, LIU Qiaoping, YAN Zhanfeng, LIU Siming, ZHU Yajing, DING Qian, ZHANG Ying, TIAN Yuan, ZHANG Jingran   

  1. Department of Otolaryngology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China
  • Published:2021-09-29

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Abstract: Objective To analyze the mechanism of action of the Xiaoqinglong decoction in allergic rhinitis treatment based on network pharmacology in order to provide direction and reference for its clinical application and further research. Methods Multiple data platforms were used to analyze the mechanism of action of the Xiaoqinglong decoction in allergic rhinitis. Through the screening of OB and DL values in the TCMSP database, the effective chemical components of the decoction, as well as their action targets, were obtained. The Cytoscape 3.7.1 software was used to draw the network diagram of the decoction's effective component-target and screening the drug's key components. Meanwhile, potential allergic rhinitis targets were obtained using the GenCards and OMIM databases, and the different genes were obtained from the GEO database and were included in the next analysis. Using an online drawing software, a Venn diagram was drawn to extract the intersection targets of the Xiaoqinglong decoction and allergic rhinitis disease targets for further analysis. Protein interaction analysis was then carried out for the intersection targets on the String platform for PPI network construction, which was imported into the Cytoscape3.7.1 software to screen the decoction core targets in allergic rhinitis treatment. The Metascape platform was used to analyze the biological processes and pathways involved in the intersection targets of Xiaoqinglong decoction-allergic rhinitis, and the GO and KEGG analyses data were obtained afterwards. The OriginPro2020 was then used to visualize the results obtained and analyzed using the Cytoscape 3.7.1 software, constructing the “Xiaoqinglong Decoction-allergic rhinitis intersection targets-signal pathway” diagram in the process. Additionally, AutoDock was used for molecular docking verification of these key components and core targets. Results The effective chemical components of the Xiaoqinglong decoction in allergic rhinitis treatment included quercetin, kaempferol, and naringenin, among others. Meanwhile, the identified core targets were STAT3, IL6, and TNF, among others. Moreover, the signaling pathway of the Xiaoqinglong decoction in allergic rhinitis treatment mainly acted on the TNF, MAPK, PI3K-Akt, IL-17, and HIF signaling pathways, wherein the molecular function was mainly found to mediate receptor ligand and receptor regulator activities, among others. Conclusion This study initially revealed the multi-component, multi-targeted, and multi-pathway mechanisms of action the Xiaoqinglong decoction in allergic rhinitis treatment, providing references for the drug's clinical applications.

Key words: Network pharmacology, Molecular docking, Allergic rhinitis, Xiaoqinglong Decoction

CLC Number: 

  • R765.21
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