JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2018, Vol. 32 ›› Issue (3): 47-53.doi: 10.6040/j.issn.1673-3770.0.2018.068

Previous Articles     Next Articles

BRF2 in the pathogenesis of chronic rhinosinusitis with nasal polyps

GAN Bin1, ZHANG Yongju1, XU Anting2   

  1. Department of Otorhinolaryngology Head and Neck Surgery, Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2018-01-27 Online:2018-05-20 Published:2018-05-20

PDF (PC)

318

Abstract: Objective To study the expression and significance of BRF2 in chronic rhinosinusitis with nasal polyps. Methods 37 surgical specimens of chronic rhinosinusitis with nasal polyps(CRSwNP)and 18 surgical specimens of simple deviation of nasal septum were collected from 2016 to 2017 in the Department of Otorhinolaryngology Head and Neck Surgery, Second Hospital of Shandong University. Immunofluorescence staining was used to detect the expression and localization of BRF2 in chronic rhinosinusitis with nasal polyps. After cultured human nasal mucosa epithelial cells were stimulated with Llipopolysaccharides(LPS)in vitro, the expression and localization of BRF2 in cells were detected by immunofluorescence staining. Western blotting and quantitative real-time PCR were used to detect the expression of BRF2 and the endoplasmic reticulum stress-related protein GRP78 in cells. Results The expression of BRF2 in chronic rhinosinusitis with nasal polyps was up-regulated in whole cells and was particularly increased in the cytoplasm fraction. In vitro, LPS stimulation can induce the up-regulation of expression of BRF2 to be up-regulated in nasal mucosal epithelial cells and increase the distribution of BRF2 in the cytoplasm, and tThe expression of endoplasmic reticulum stress-related protein GRP78 was also increased. Conclusion BRF2 may mediate endoplasmic reticulum stress, which is involved in the pathogenesis of chronic rhinosinusitis with nasal polyps.

Key words: Human nasal epithelial cells, BRF2, GRP78, Nasal polyps, Rhinosinusitis, chronic

CLC Number: 

  • R765.4+1
[1] Akdis CA, Bachert C, Cingi C, et al. Endotypes and phenotypes of chronic rhinosinusitis: a PRACTALL document of the European Academy of Allergy and Clinical Immunology and the American Academy of Allergy, Asthma & Immunology[J]. J Allergy Clin Immunol, 2013, 131(6): 1479-90.
[2] Mu(~overn)oz-Del-Castillo F, Jurado-Ramos A, Soler R, et al. Fungal sensitization in nasal polyposis[J]. J Investig Allergol Clin Immunol, 2009, 19(1): 6-12.
[3] Cabarcas S, Schramm L. RNA polymerase III transcription in cancer: the BRF2 connection[J]. Mol Cancer, 2011. 10: 47.
[4] Moreno-Moya JM, Vilella F, Simón C. MicroRNA: key gene expression regulators[J]. Fertil Steril, 2014, 101(6): 1516-1523.
[5] Lockwood WW, Chari R, Coe BP, et al. Integrative genomic analyses identify BRF2 as a novel lineage-specific oncogene in lung squamous cell carcinoma[J]. PLoS Med, 2010, 7(7): e1000315.
[6] Cabarcas S, Jacob J, Veras I, Schramm L. Differential expression of the TFIIIB subunits Brf1 and Brf2 in cancer cells[J]. BMC Mol Biol, 2008, 9: 74.
[7] Gullo F, Ceriani M, D'Aloia A, et al. Plant polyphenols and exendin-4 prevent hyperactivity and TNF-α release in LPS-treated in vitro neuron/astrocyte/microglial networks[J]. Front Neurosci, 2017, 11: 500.
[8] Shao L, Liu X, Zhu S, et al. The role of smurf1 in neuronal necroptosis after lipopolysaccharide-induced neuroinflammation[J]. Cell Mol Neurobiol, 2017. DOI:10.1007/s10571-017-0553-6.
[9] Lee S, Lee D, Jang TS, et al. Anti-inflammatory phenolic metabolites from the edible fungus phellinus baumii in LPS-stimulated RAW264.7 cells[J]. Molecules, 2017, 22(10). pii: E1583. DOI: 10.3390/molecules22101583
[10] Gaut JR, Hendershot LM. The modification and assembly of proteins in the endoplasmic reticulum[J]. Curr Opin Cell Biol, 1993, 5(4): 589-595.
[11] Hotamisligil GS. Endoplasmic reticulum stress and the inflammatory basis of metabolic disease[J]. Cell, 2010, 140(6): 900-917.
[12] Appenzeller-Herzog C, Hall MN. Bidirectional crosstalk between endoplasmic reticulum stress and mTOR signaling[J]. Trends Cell Biol, 2012, 22(5): 274-82.
[13] Ozcan L, Tabas I. Calcium signalling and ER stress in insulin resistance and atherosclerosis[J]. J Intern Med, 2016, 280(5): 457-464.
[14] Xiang C, Wang Y, Zhang H, et al. The role of endoplasmic reticulum stress in neurodegenerative disease[J]. Apoptosis, 2017, 22(1): 1-26.
[15] Zhang K, Kaufman RJ. From endoplasmic-reticulum stress to the inflammatory response[J]. Nature, 2008, 454(7203): 455-62.
[16] Malhotra JD, Miao H, Zhang K, et al. Antioxidants reduce endoplasmic reticulum stress and improve protein secretion[J]. Proc Natl Acad Sci U S A, 2008, 105(47): 18525-18530.
[17] Gouge J, Satia K, Guthertz N, et al. Redox signaling by the RNA polymerase III TFIIB-related factor Brf2[J]. Cell, 2015, 163(6): 1375-87.
[18] Guo H, Cui H, Peng X, et al. Nickel chloride(NiCl2)induces endoplasmic reticulum(ER)stress by activating UPR pathways in the kidney of broiler chickens[J]. Oncotarget, 2016, 7(14): 17508-17519.
[19] Lund VJ, Kennedy DW. Staging for rhinosinusitis[J]. Otolaryngol Head Neck Surg,1997, 117(3 Pt 2): 35-40.
[20] 鲁铭, 田辉, 李树海, 等. BRF2基因在食管鳞状细胞癌组织中的表达及其临床意义[J]. 中国肿瘤生物治疗杂志, 2012, 19(2): 175-179. LU Ming, TIAN Hui, LI Shuhai, et al. BRF2 gene expression in esophageal squamous cell carcinoma tissues and its clinical significance[J]. Chin J Cancer Biother, 2012, 19(2): 175-179.
[21] Tian Y, Lu M, Yue W, et al. TFIIB-related factor 2 is associated with poor prognosis of nonsmall cell lung cancer patients through promoting tumor epithelial-mesenchymal transition[J]. Biomed Res Int, 2014, 2014: 530786.
[22] Lu M, Tian H, Yue W, et al. TFIIB-related factor 2 over expression is a prognosis marker for early-stage non-small cell lung cancer correlated with tumor angiogenesis[J]. PLoS One, 2014, 9(2): e88032.
[1] SONG Xiaoyu, ZHANG Yu, LI Chenglin, SONG Xicheng. Enhanced recovery after surgery for the treatment of chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2018, 32(5): 9-12.
[2] HUANG Yuan, BA Luo, LI Bo, YU Wenxing, YANG Fangli, DU Jintao. Transcription factor NKX2-1 regulates eosinophilic inflammation in chronic rhinosinusitis with nasal polyps [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 17-23.
[3] CHEN Wangxing, ZHAO Xue, LIU Yan, LIU Yue, JIN Chunshun. Psychological status of patients with deviated nasal septum or chronic sinusitis with nasal polyps [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 24-27.
[4] Effect of short-term glucocorticoid inhalation on nasal symptoms and adrenocortical function in patients with eosinophilic chronic sinusitis accompanied by nasal polyps. Effect of short-term glucocorticoid inhalation on nasal symptoms and adrenocortical function in patients with eosinophilic chronic sinusitis accompanied by nasal polyps [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 28-31.
[5] LI Huabin, LAI Yuting. Pathogenesis, diagnosis, and treatment of chronic rhinosinusitis [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(3): 4-9.
[6] YU Qianqian, GUAN Ge, ZHANG Niankai, LI Na, WEN Junfeng, LIU Tingting, LIAN Yuanyuan. Characteristics of respiratory epithelial adenomatoid hamartomas in the nasal olfactory cleft on computed tomography [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(3): 76-81.
[7] LI Hongmei, WANG Lan, GAO Zhaohui, WANG Ying, XUE Jinmei. The expression and significance of p-ERK2/ ERK2 in nasal polyps [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(3): 42-46.
[8] ZHANG Jie, GONG Qi. Clinical significance of eosinophils and neutrophils in blood and allergic reaction in patients with chronic rhinosinusitis [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(3): 54-57.
[9] ZHI Lili, SONG Daoliang. Expressions of eosinophils and IL5 in antrochoanal polyps and nasal polyps. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(4): 43-46.
[10] LUN Jie, ZHANG Ya′nan. Analysis on the factors related to the recurrence of chronic rhinosinusitis with nasal polyps. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(3): 80-83.
[11] WANG Haipeng, XU Huijuan, ZHANG Xiufang, WANG Huizhong, LI Yanzhong. The application of Sinus Jel in adult patients of nasal polyps with sleep-disordered breathing. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(5): 58-60.
[12] SU Yi, XIE Jinghua, PAN Xiaoli, ZENG Guoqing. Efficacy and associated factors of endoscopic sinus surgery on patients with chronic rhinosinusitis with or without nasal polyps. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(4): 86-89.
[13] LI Houjie, QU Qian, XU Shicai, LIU Lingbing, ZHANG Yongchao, WANG Xuzeng, LIU Lei. Clinical experience on the treatment of antrochoanal polyp under nasal endoscopy by expand road of the combined middle-inferior meatus. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(2): 62-64.
[14] ZHANG Jian, LU Xiaoqing, WU Jian.. Differentially expressed proteins between nasal polyps and normal nasal mucosa by the iTRAQ technology. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(1): 5-8.
[15] DU Zhihong, YU Yafeng.. Effect of NLRP3 inflammasome in the pathogenesis and relapse of eosinophilic nasal polyps. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(1): 31-35.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] ZHAO Li-min, NI Kun, WU Jia-li, CHEN Shu-mei, LI Xiao-yan. Etiology and treatment of inspiratory laryngeal stridor in infants[J]. J Otolaryngol Ophthalmol Shandong Univ, 2013, 27(2): 49 -51 .
[2] SHI Chun-sheng1, ZHANG Qing-quan2， WANG Qiang2, SUN Yan2, GE Chang-yi1. Application of flaps in the reconstruction of soft palate defects[J]. J Otolaryngol Ophthalmol Shandong Univ, 2013, 27(2): 86 -88 .
[3] ZHANG Ling1, SUN Chuan-yi1, XU An-ting2. 1 case of petrous apex cholesteatoma deafness patients[J]. J Otolaryngol Ophthalmol Shandong Univ, 2013, 27(2): 93 -94 .
[4] . [J]. J Otolaryngol Ophthalmol Shandong Univ, 2013, 27(4): 1 -3 .
[5] LUO Hui-xiu, FAN Chun-tao, DENG Yan-hua. Therapeutic effects of adenoidectomy and tonsillectomy in children with obstructive sleep apnea hypopnea syndrome[J]. J Otolaryngol Ophthalmol Shandong Univ, 2013, 27(4): 17 -20 .
[6] HAO Ying-juan1, YANG Qing-song2, ZHOU Yue-hua2, YI Sheng-ping1， ZHAI Chang-bin2. Characteristics of thin corneal flaps created with Wavelight FS200 femtosecond laser[J]. J Otolaryngol Ophthalmol Shandong Univ, 2013, 27(4): 26 -31 .
[7] WEN Hua-ming1, LI Hai-xiang2. Application of trianglar function method to calculate intra ocular lens power after myopic laser in situ keratomileusis[J]. J Otolaryngol Ophthalmol Shandong Univ, 2013, 27(5): 74 -76 .
[8] ZHU Jian， HE Qing-qing， ZHENG Lu-ming， FAN Zi-yi， ZHAO Guo-wei， HOU Lei， SHI Hou-bin. Clinical analysis for 57 re-operational cases of differentiated thyroid cancer[J]. J Otolaryngol Ophthalmol Shandong Univ, 2013, 27(6): 9 -12 .
[9] LI Shao-hua, SUN Yi-fan, LU Biao-qing. Application of double vascularized soft tissue flap in open radical mastoidectomy[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(1): 6 -7 .
[10] WEI Xi-jian1, YIN Rui-lan2, GUO Xing3. Expression of a proliferation-inducing ligand in laryngeal squamous cell carcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(1): 14 -16 .
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd