Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2021, Vol. 35 ›› Issue (1): 40-46.doi: 10.6040/j.issn.1673-3770.0.2020.279

Previous Articles     Next Articles

Agreement and association analyses of skin prick testing and serum specific IgE assay for aeroallergen detection in patients with allergic rhinitis from Jiangsu and Anhui areas

YANG Qing1, LU Meiping1,2, CHENG Lei1,2,3   

  1. 1. Department of Otorhinolaryngology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, Jiangsu, China;
    2. Clinical Allergy Center, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, Jiangsu, China;
    3. International Centre for Allergy Research, Nanjing Medical University, Nanjing 210029, Jiangsu, China
  • Published:2021-02-01

PDF (PC)

395

Abstract: Objective To assess the agreement and association between skin prick testing(SPT)and serum specific IgE(sIgE)in detecting common aeroallergens in patients with allergic rhinitis(AR). Methods The study included 101 patients(53 males and 48 females; age range: 4 to 68 years, average age: 38.5±14.8 years)from the Jiangsu and Anhui Provinces who received treatment for AR in the First Affiliated Hospital of Nanjing Medical University from November 2018 to December 2019. All the patients simultaneously underwent SPT and sIgE for the detection of 10 aeroallergens; these included 6 perennial indoor allergens(Dermatophagoides farina, Dermatophagoides pteronyssinus, cat dander, dog dander, Blattella germanica, and Alternaria alternate)and 4 seasonal pollen allergens(birch, ragweed, humulus, and Artemisia ［A. annua or A. argyi］). Meanwhile, the results of the allergen detection were analyzed by SPSS 24.0 software. Results The agreement percentages of the detections of D. farina, D. pteronyssinus, A. alternata, cat dander, dog dander, humulus, B. germanica, ragweed, Artemisia, and birch by SPT and sIgE were 75.28%, 72.09%, 39.13%, 36.84%, 11.11%, 14.29%, 12.50%, 22.22%, 13.33%, and 15.38%, respectively. Cohen's kappa statistics showed that the κ-coefficients were between 0.146 and 0.484. Among them, the κ-coefficients of A. alternata, cat dander, and D. farina were 0.484, 0.471, and 0.413, respectively, which indicated moderate agreements. The κ-coefficients of D. pteronyssinus, ragweed, and birch were 0.398, 0.330, and 0.215, respectively, which indicated lower agreements. All the κ-coefficients of the other four allergens were less than 0.20, indicating poor agreement. Spearman's rank correlation analysis showed positive correlations between the SPT and sIgE results for the detection of the 10 aeroallergens(rs ranged from 0.174 to 0.548). The rs values of D. farina, A. alternata, cat dander, and D. pteronyssinus were 0.548, 0.532, 0.483, and 0.440, respectively, indicating moderate correlations. The rs values of humulus, birch, and dog dander were 0.240, 0.239, and 0.210, respectively, indicating poor correlations. The rs values of the other three allergens were all < 0.20, indicating very poor correlation. Conclusion This study demonstrated large discrepancies in the agreement and association between the SPT and sIgE detections of common aeroallergens in patients with AR in the Jiangsu and Anhui areas. The combination of in vivo and in vitro methods for allergen testing is recommended to improve the accuracy of the clinical diagnosis of AR.

Key words: Allergic rhinitis, Allergens, Skin prick test, Specific IgE, Agreement, Association

CLC Number: 

  • R765.21
[1] Douglass JA, O'Hehir RE. 1. Diagnosis, treatment and prevention of allergic disease: the basics[J]. Med J Aust, 2006, 185(4):228-233. doi: 10.5694/j.1326-5377.2006.tb00539.x
[2] Bro(·overz)ek JL, Bousquet J, Agache I, et al. Allergic Rhinitis and its Impact on Asthma(ARIA)guidelines-2016 revision[J]. J Allergy Clin Immunol, 2017, 140(4):950-958. doi: 10.1016/j.jaci.2017.03.050.
[3] Krouse JH, Mabry RL. Skin testing for inhalant allergy 2003: current strategies[J]. Otolaryngol Head Neck Surg, 2003, 129(Suppl 4):33-49. doi: 10.1016/S0194-5998(03)01398-6.
[4] Gendo K, Larson EB. Evidence-based diagnostic strategies for evaluating suspected allergic rhinitis[J]. Ann Intern Med, 2004, 140(4):278-289. doi: 10.7326/0003-4819-140-4-200402170-00010.
[5] Szeinbach SL, Harpe SE, Williams PB, et al. Testing for allergic disease: parameters considered and test value[J]. BMC Fam Pract, 2008, 9(1):47. doi: 10.1186/1471-2296-9-47
[6] Bulat Lokas S, Plavec D, RikicPiskoviic J, et al. Allergen-Specific IgE Measurement: Intermethod Comparison of Two Assay Systems in Diagnosing Clinical Allergy[J]. J Clin Lab Anal, 2017, 31(3):e22047. doi: 10.1002/jcla.22047.
[7] Ansotegui IJ, Melioli G, Canonica GW, et al. IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper[J]. World Allergy Organ J, 2020, 13(2):100080.. doi: 10.1016/j.waojou.2019.100080.
[8] 钱俊俊, 周文成, 吴中飞, 等. 苏皖地区变应性鼻炎患者吸入性变应原谱分析[J]. 山东大学耳鼻喉眼学报, 2019, 33(3):147-151. doi:10.6040/j.issn.1673-3770.1.2019.036. QIAN Junjun, ZHOU Wencheng, WU Zhongfei, et al. Analysis of sensitization to inhalant allergens in patients with allergic rhinitis from Jiangsu and Anhui areas[J]. Journal of Qtolaryngology and Ophthalmology of Shandong University, 2019, 33(3):147-151. doi:10.6040/j.issn.1673-3770.1.2019.036.
[9] 中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组, 中华医学会耳鼻咽喉头颈外科学分会鼻科学组. 变应性鼻炎诊断和治疗指南(2015年, 天津)[J]. 中华耳鼻咽喉头颈外科杂志, 2016, 51(1):6-24. doi:10.3760/cma.j.issn.1673-0860. 2016.01.004.
[10] Schoos AMM, Chawes BLK, Følsgaard NV, et al. Disagreement between skin prick test and specific IgE in young children[J]. Allergy, 2015, 70(1):41-48. doi: 10.1111/all.12523.
[11] Knight V, Wolf ML, Trikha A, et al. A comparison of specific IgE and skin prick test results to common environmental allergens using the HYTECTM 288[J]. J Immunol Methods, 2018, 462:9-12. doi: 10.1016/j.jim.2018.07.005.
[12] Cho JH, Suh JD, Kim JK, et al. Correlation between skin-prick testing, individual specific IgE tests, and a multiallergen IgE assay for allergy detection in patients with chronic rhinitis[J]. Am J Rhinol Allergy, 2014, 28(5):388-391. doi:10.2500/ajra.2014.28.4074.
[13] Srisuwatchari W, Kwanthong P, Bunnag C, et al. Association between skin prick test and serum specific immunoglobulin E to American cockroach allergens in allergic rhinitis patients[J]. Allergol Immunopathol(Madr), 2020, 48(2):170-174. doi: 10.1016/j.aller.2019.07.007.
[14] Chauveau A, Dalphin ML, Mauny F, et al. Skin prick tests and specific IgE in 10-year-old children: Agreement and association with allergic diseases[J]. Allergy, 2017, 72(9):1365-1373. doi:10.1111/all.13148.
[15] Chu HT, Godin I, Phuc'n'g NT, et al. Evaluation of skin prick test to screen dust mite sensitization in chronic respiratory diseases in Southern Vietnam[J]. Asia Pac Allergy, 2018, 8(4):e39. doi:10.5415/apallergy.2018.8.e39.
[16] Wagner N, Rudert M. Sensitivity and specificity of standardised allergen extracts in skin prick test for diagnoses of IgE-mediated respiratory allergies[J]. Clin Transl Allergy, 2019, 9:8. doi:10.1186/s13601-019-0248-9.
[17] Wang J, Godbold JH, Sampson HA. Correlation of serum allergy(IgE)tests performed by different assay systems[J]. J Allergy Clin Immunol, 2008, 121(5):1219-1224. doi: 10.1016/j.jaci.2007.12.1150.
[18] Siles RI, Hsieh FH. Allergy blood testing: A practical guide for clinicians[J]. Cleve Clin J Med. 2011;78(9):585-592. doi:10.3949/ccjm.78a.11023.
[19] Cox L. Overview of serological-specific IgE antibody testing in children[J]. Curr Allergy Asthma Rep. 2011;11(6):447-453. doi:10.1007/s11882-011-0226-3.
[20] Calabria CW, Dietrich J, Hagan L. Comparison of serum-specific IgE(ImmunoCAP)and skin-prick test results for 53 inhalant allergens in patients with chronic rhinitis[J]. Allergy Asthma Proc, 2009, 30(4):386-396. doi:10.2500/aap.2009.30.3258.
[21] Cobbaert CM, Jonker GJ. Allergy testing on the IMMULITE 2000 Random-Access immunoanalyzer—a clinical evaluation study[J]. Clin Chem Lab Med, 2005, 43(7):772-781. doi:10.1515/CCLM.2005.132.
[22] Frith J, Fleming L, Bossley C, et al. The complexities of defining atopy in severe childhood asthma[J]. Clin Exp Allergy, 2011, 41(7):948-953. doi: 10.1111/j.1365-2222.2011.03729.x.
[23] Ollert M, Weissenbacher S, Rakoski J, Ring J. Allergen-specific IgE measured by a continuous random-access immunoanalyzer: interassay comparison and agreement with skin testing[J]. Clin Chem, 2005, 51(7):1241-1249. doi: 10.1373/clinchem.2004.046565.
[24] Ciprandi G, De Amici M, Giunta V, et al. Comparison of serum specific IgE and skin prick test in polysensitized patients[J]. Int J Immunopathol Pharmacol, 2010, 23(4):1293-1295. doi:10.1177/039463201002300438.
[25] Park KH, Lee J, Lee SC, et al. Comparison of the ImmunoCAP Assay and AdvanSureTM AlloScreen Advanced Multiplex Specific IgE Detection Assay[J]. Yonsei Med J, 2017, 58(4):786-792. doi: 10.3349/ymj.2017.58.4.786.
[26] 付婉艺. 中国蒿属花粉过敏原分子生物学分析和组分诊断[D]. 浙江大学, 2018.
[27] Katial RK, Lin FL, Stafford WW, et al. Mugwort and sage(Artemisia)pollen cross-reactivity: ELISA inhibition and immunoblot evaluation[J]. Ann Allergy Asthma Immunol, 1997, 79(4):340-346. doi:10.1016/s1081-1206(10)63025-6.
[1] CHENG Lei, XU Qiuyan, CHEN Hao. Clinical use and significance of allergy testing and diagnostics [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 1-6.
[2] NI Jingzi, WAN Wenjin,CHENG Lei. Research progress on health-related quality of life in allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 110-115.
[3] LIN Yihang,LI Youjin. Research progress on gut microbiome in children with allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 116-122.
[4] LIU Zhen,SONG Xicheng. Mechanisms and research progress of pyroptosis in allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 123-129.
[5] LIU Yitong, ZHOU Suizi,QIU Qianhui. Research progress on NLRP3 inflammasome in chronic rhinosinusitis and allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 142-146.
[6] GONG Xiaoyang, CHENG Lei. Analysis of proportion of outpatients with allergic rhinitis during the coronavirus infectious disease 2019 pandemic [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 245-255.
[7] ZHANG Yaqi, LIU Huimin, CAO Linman, WANG Ziyu, LIN Xu, LI Yanping, XUE Gang, WU Jingfang. Expression and significance of the MAPK,PI3K-AKT,NF-κB pathways of allergic rhinitis in mice [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 254-259.
[8] LU Weili, JIANG Tao, LI Xianhua. Analysis of sIgE in polysensitized children with allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 260-265.
[9] HUANG Kaiyue, LI Xueqing, HAN Gouxin, ZHANG Qinxiu. Meta-analysis of acupoint catgut embedding in the treatment of allergic rhinitis based on the theory of “lung and spleen” [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 266-274.
[10] ZHU Zhengru, ZHANG Xiaobing. Meta-analysis of the curative effect of traditional Chinese medicine decoction combined with conventional western medicine on allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 281-289.
[11] PANG Chong, BIAN Sainan, ZHANG Bing, YIN Xu, LU Yingxia, YE Pengfei, WANG Zhan, ZHAO Jing, GAO Yan, GUAN Kai. Short-term effect of Dermatophagoides farinae specific sublingual immunotherapy for children with allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(1): 70-74.
[12] WANG Fei, LIU Yuying, XIAO Qiyi, DING Jian, GAO Shang, MAO Wei. A study on the associations between psychological factors and allergic rhinitis among residents [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(5): 28-31.
[13] WANG Xin, LIU Qiaoping, YAN Zhanfeng, LIU Siming, ZHU Yajing, DING Qian, ZHANG Ying, TIAN Yuan, ZHANG Jingran. A study on the mechanism of action of the Xiaoqinglong Decoction in treating allergic rhinitis based on network pharmacology [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(5): 46-55.
[14] WANG Yuting，,WANG Jiaxi. Research developments on microRNA in the pathogenesis of allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(5): 98-104.
[15] LI Huajing, HAO Runmei, DAI Hao, ZHANG Ling, SHEN Zhen, QUAN Fang, SHAO Yuan. Mechanism of inhibition of ovalbumin-induced inflammation by catechins in an OVA-induced mouse model of allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(4): 1-7.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(1): 84 -87 .
[2] NIU Shanli,CHAI Maowen,LI Zhenxiu . Endoscopic rhinoplasty of inferior turbinate in 60 patients with chronic hypertrophic rhinitis[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(1): 16 -18 .
[3] MENG Qing-guo,LU Yong-tian,FAN Xian-liang .

Association of killer cell immunoglobulin-like receptor gene polymorphisms with nasopharyngeal carcinoma

[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 196 -199 .
[4] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 199 -199 .
[5] WAN Li-jia,LU Hai-tao,JIANG Yi-dao,LIU Hui,LI Qin,SHE La-zhi . Effect of H-uvulopalatopharyngoplasty on obstructive sleep apnea
hypopnea syndrome[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 204 -205 .
[6] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 222 -224 .
[7] JI Xiao-bin,DENG Jia-de,ZANG Lin-quan,WANG Lei,XIE Jun . Blood histamine in guinea pigs with allergic rhinitis[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 228 -230 .
[8] XIANG Deng,LU Yong-tian,SUN Huan-ji . Endoscopic repair for cerebrospinal fluid rhinorrhea in 19 cases and a literature review [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 234 -236 .
[9] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 253 -257 .
[10]
YIN Guo-hua,ZHONG Xiao . Long-term effect of laser reduction on lingua adenoids
[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 280 -282 .
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd