山东大学耳鼻喉眼学报 ›› 2018, Vol. 32 ›› Issue (5): 66-70.doi: 10.6040/j.issn.1673-3770.0.2018.105

• ·论著· • 上一篇    下一篇

变应性鼻炎大鼠IL-35的表达及对辅助性T细胞免疫调节的影响

卢汉桂,林歆胜,姚丹勉,魏永新,李创伟   

  1. A2013653)第一作者:卢汉桂。 E-mail:sandro_gui@126.comDOI:10.6040/j.issn.1673-3770.0.2018.105变应性鼻炎大鼠IL-35的表达及对辅助性T细胞免疫调节的影响卢汉桂, 林歆胜, 姚丹勉, 魏永新, 李创伟(汕头市中心医院耳鼻咽喉头颈外科, 广东 汕头 515031
  • 收稿日期:2018-02-28 出版日期:2018-09-20 发布日期:2018-09-20
  • 基金资助:
    广东省医学科学技术研究基金项目(A2013653)

Expression of IL-35 in allergic rhinitis and its effect on the immunoregulation of T cells

LU Hangui, LIN Xinsheng, YAO Danmian, WEI Yongxin, LI Chuangwei   

  1. Department of Otorhinolaryngology Head and Neck Surgery, Shantou Central Hospital, Shantou 515031, Guangdong, China
  • Received:2018-02-28 Online:2018-09-20 Published:2018-09-20

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摘要： 目的 探讨变应性鼻炎(AR)大鼠IL-35的表达及对辅助性T细胞免疫调节的影响。 方法 以卵清蛋白作为致敏原,建立AR大鼠模型。大鼠随机分为对照组、模型组、地塞米松组和IL-35干预组。分析比较大鼠过敏症状评分;采用RT-PCR分析鼻黏膜中IL-35、IFN-γ、IL-4和IL-17的mRNA表达情况;酶联免疫吸附法测定IL-35、IFN-γ、IL-4和IL-17在外周血的表达水平;流式细胞术检测外周血中Th1、Th2和Th17细胞百分比。 结果 IL-35干预可明显降低AR大鼠的症状积分;AR模型组IL-35和IFN-γ的mRNA表达及在外周血的表达均显著低于对照组,而在IL-35干预组的表达高于AR模型组,AR模型组IL-4和IL-17表达显著高于对照组,而在IL-35干预组的表达低于AR模型组(P<0.05);AR模型组Th1细胞百分比显著低于对照组,而在IL-35干预组Th1细胞百分比显著高于AR模型组,Th2和Th17细胞百分比显著高于对照组,IL-35干预组Th2和Th17细胞百分比显著低于AR模型组(P<0.05)。 结论 AR大鼠IL-35的表达降低,IL-35干预可通过上调IFN-γ和下调IL-4、IL-17表达降低炎症反应,机制可能与调节Th1、Th2和Th17平衡有关。

关键词: 辅助性T细胞, 变应性鼻炎, IL-35

Abstract: Objective To investigate the expression of IL-35 in allergic rhinitis(AR)and to explore its effect on the immunoregulation of T cells. Methods A rat model of AR was established by using ovalbumin as the allergen. Rats were randomly divided into the control group, model group, dexamethasone group, and IL-35 intervention group. Anaphylaxis score of rats was analyzed and compared. RT-PCR was used to analyze the mRNA expressions of IL-35, IFN-γ, IL-4, and IL-17 in the nasal mucosa. The expression levels of IL-35, IFN-γ, IL-4, and IL-17 in peripheral blood were measured by enzyme linked immunosorbent assay. Flow cytometry was used to detect the percentage of Th1, Th2, and Th17 cells in peripheral blood. Results IL-35 intervention can significantly reduce the symptoms of AR rats. The expression levels of IL-35 and IFN-γ in the AR model group and in peripheral blood were significantly lower than in the control group, whereas the expression levels were higher in the IL-35 intervention group than in the AR group. IL-4 and IL-17 expression levels were significantly higher in the AR model group than in the control group, whereas the expression levels were lower in the IL-35 intervention group than in the AR model group. The percentage of Th1 cells in the AR model group was significantly lower than that in the control group, whereas the percentage of Th1 cells in the IL-35 intervention group was significantly higher than that in the AR model group. The percentage of Th2 and Th17 cells was significantly higher in the AR model group than that in the control group. The percentage of Th2 and Th17 cells in the IL-35 intervention group was significantly lower than that in the AR model group(P<0.05). Conclusion IL-35 expression was downregulated in AR rats. IL-35 intervention can reduce the inflammatory response by upregulation of IFN-γ and downregulation of IL-4 and IL-17. The mechanism may be related to the regulation of Th1, Th2, and Th17 balance.

Key words: IL-35, Helper T cells, Allergic rhinitis

中图分类号: 

  • R765.4
[1] Seo JH, Kim HY, Jung YH, et al. Interactions between innate immunity genes and early-life risk factors in allergic rhinitis[J]. Allergy, Asthma Immunol Res, 2015, 7(3):241-248.
[2] Yokota M, Suzuki M, Nakamura Y, et al. Cytokine modulation by IL-35 in mice with allergic rhinitis[J]. Am J Rhinol Allergy, 2015, 29(4):251-256.
[3] Shi Y, Dai M, Wu G, et al. Levels of interleukin-35 and its relationship with regulatory T-cells in chronic hepatitis B patients[J]. Viral Immunol, 2015, 28(2):93-100.
[4] Vaughan RA, Gannon NP, Mermier CM, et al. Irisin, a unique non-inflammatory myokine in stimulating skeletal muscle metabolism[J]. J Physiol Biochem, 2015, 71(4):679-689.
[5] 安云芳, 赵长青. 变应性鼻炎鼻黏膜P物质受体的研究[J]. 中华耳鼻咽喉科杂志, 1998, 33(3):139-142. AN Yunfang, ZHAO Changqing. Distribution of substance P receptors in rat nasal mucosa of allergic rhinitis[J]. Chin J Otolaryngol, 1998, 33(3):139-142.
[6] 中华医学会耳鼻咽喉科学分会. 变应性鼻炎诊断标准及疗效评定标准[J]. 中华耳鼻咽喉科杂志,1998,33(3):134-135.
[7] Dixon KO, der Kooij SW, Vignali DAA, et al. Human tolerogenic dendritic cells produce IL-35 in the absence of other IL-12 family members[J]. Eur J Immunol, 2015, 45(6):1736-1747.
[8] Ozkan ZS, Simsek M, Ilhan F, et al. Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-βlevels in pregnant women with preeclampsia, and their relation with severity of disease[J]. J Mater Fetal Neonatal Med, 2014, 27(15):1513-1517.
[9] Dong J, Wong CK, Cai Z, et al. Amelioration of allergic airway inflammation in mice by regulatory IL-35 through dampening inflammatory dendritic cells[J]. Allergy, 2015, 70(8):921-932.
[10] Shen P, Roch T, Lampropoulou V, et al. IL-35-producing B cells are critical regulators of immunity during autoimmune and infectious diseases[J]. Nature, 2014, 507(7492):366-370.
[11] Kochetkova I, Thornburg T, Callis G, et al. Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27[J]. J Immunol, 2014, 192(2):804-816.
[12] Zongyi Y, Funian Z, Hao L, et al. Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio[J]. PloS one, 2017, 12(12):e0189617.
[13] 刘仁慧, 王秀娟, 许利平, 等. 培本中药及其拆方调节哮喘大鼠Th1/Th2平衡的实验研究[J]. 北京中医药大学学报, 2014, 37(3):165-170. LIU Renhui, WANG Xiujuan, XU Liping, et al. Peiben Fang and its disassembled formulas for regulating Th1/Th2 balance in rats with asthma[J]. J Beijing Univ Trad Chin Med, 2014, 37(3):165-170.
[14] Gu ZW, Wang YX, Cao ZW. Neutralization of interleukin-9 ameliorates symptoms of allergic rhinitis by reducing Th2, Th9, and Th17 responses and increasing the Treg response in a murine model[J]. Oncotarget, 2017, 8(9):14314-14324.
[15] Wong CK, Leung TF, Chu I MT, et al. Aberrant expression of regulatory cytokine IL-35 and pattern recognition receptor NOD2 in patients with allergic asthma[J]. Inflammation, 2015, 38(1):348-360.
[16] 唐瑜健. IL-35 蛋白对小鼠铜绿假单胞菌肺炎的干预作用[D]. 泸州:四川医科大学, 2015.
[17] Yin X, Ren X, Liu C, et al. The regulatory effect of IL-35 on the balance of Treg/Th17 cells in allergic rhinitis patients[J]. J Clin Otorhinolaryngol Head Neck Surgery, 2016, 30(3):213-216.
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