体外培养人胚晶状体上皮细胞生长特性的研究

山东大学耳鼻喉眼学报

体外培养人胚晶状体上皮细胞生长特性的研究

邹俊¹，卢奕²，褚仁远²

1．上海交通大学附属第六人民医院眼科，上海 200233；2．复旦大学附属眼耳鼻喉科医院眼科，上海 200031

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-10-16 发布日期:2008-10-16
通讯作者: 邹俊

Growth features of human embryonic lens epithelial cells cultured in vitro

ZOU Jun¹, LU Yi², CHU Ren-yuan²

1. Department of Ophthalmology, Shanghai Sixth People′s Hospital, Shanghai Jiaotong University, Shanghai 200233, China;
2. Department of Ophthalmology, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai 200031, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-10-16 Published:2008-10-16
Contact: ZOU Jun

摘要/Abstract

摘要： 目的观察体外培养人胚晶状体上皮细胞的生物学特性。方法组织块贴壁法培养人胚晶状体上皮细胞，通过倒置相差显微镜、透射电子显微镜，采用免疫细胞化学法分析人胚晶状体上皮细胞的生长特性。结果体外培养的原代人胚晶状体上皮细胞在组织块贴壁48～72ｈ后从组织块边缘长出，具有上皮细胞的形态特点，第10天达到融合。第二代人胚晶状体上皮生长曲线近“S”形，经过1～2ｄ的潜伏期后进入对数生长期，需10d左右达到融合，细胞形态呈六角形或椭圆形、超微结构保持正常，免疫细胞化学SABC法染色结果显示细胞浆内α－晶状体蛋白染色阳性。结论人胚晶状体上皮细胞具有增殖能力，第二代人胚晶状体上皮细胞是进行白内障相关研究的合适实验对象。

关键词: 晶状体, 上皮细胞, 细胞培养, 人胚

Abstract: To investigate the biological characteristics of human embryonic lens epithelial cells cultured in vitro. MethodsHuman embryonic lens epithelial cells were cultured by the explanting culture methods. An inverted phase contrast microscope, electron microscope and immunocytochemistry were used to study the growth specialties of the human embryonic lens epithelial cells. ResultsHuman embryonic lens epithelial cells of primary culture in vitro grew out of the edge of tissue pieces after incubation for 48-72 hours. The primary cultured cells maintained typical morphology of the epithelial cells and reached a confluence in 10 days. Growth curves of cultured human embryonic lens epithelial cells in passage 2 showed an S-shape. After a 1-2 day latent period, the cultured cells logarithmically grew and reached a confluence in 10 days. The shape of human embryonic lens epithelial cells in passage 2 was hexagon or oval with normal ultra-structure. Positive staining of α-crystalline was shown in passage 2 cultured human embryonic lens epithelial cells by immunocytochemistry. ConclusionHuman embryonic lens epithelial cells have proliferative capacity and are suitable for related study of cataracts.

Key words: Epithelial cell, Cell culture, Lens, Human, embryo

中图分类号:

R776.1

邹俊,卢奕,褚仁远 . 体外培养人胚晶状体上皮细胞生长特性的研究[J]. 山东大学耳鼻喉眼学报, 2008, 22(5): 453-456 .

ZOU Jun,LU Yi,CHU Ren-yuan . Growth features of human embryonic lens epithelial cells cultured in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(5): 453-456 .

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